Adipose Tissue-Secreted Factors Alter Bladder Cancer Cell Migration

Figure 5

Blocking or depleting IL6, CXCL1, and PAI1 reduces migration of T24 cells. (a) Blocking the activity of exogenously added recombinant IL6, CXCL1, and PAI1 with neutralizing antibodies significantly reduces T24 migration in a Transwell assay compared to treatment with an isomeric IgG control. One-way ANOVA with uncorrected Fisher’s LSD test: are statistically significant. (b) Depletion of IL6, PAI1, and CXCL1 in an obese patient ASC CM was performed by treating with neutralizing antibodies. T24 migration was assessed in a Transwell system, and migrated cells were stained and counted. One-way ANOVA: are statistically significant.

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