Angiogenesis in Non-Obese Lipedema leg fat. (a) H&E stain. Arrows indicate blood vessel lumens around adipocytes. (b, c) Immunohistochemistry of CD31 and SMA confirms the blood vessel origin of the tissue structures. (d) Trichrome stain demonstrating fibrosis in the areas of increased blood vessels. (e, f) Immunohistochemistry of CD68-labeled macrophages and CD117-labeled mast cells (arrows). Images captured at 40x objective lens (scale bar: 100 μm).