Research Article
A Case-Control Study of the Association of Leptin Gene Polymorphisms with Plasma Leptin Levels and Obesity in the Kerala Population
Table 1
List of selected SNPs of Leptin gene investigated in this study and the respective primers used for amplification of the regions of interest.
| | Primer ID No. | SNP/db SNP ID | Region | Primers (5′—3′) | Tm | Flanking sequences |
| | 1 | −2548A/G rs7799039 | Promoter-1 | F-TGGGCATCCCCACAGACGGA | 75.4 | AGGGTTGC[A/G]CTGATCCT | | R-TCACAGTGGTCCTGAGGTGACG | 69.9 |
| | 2 | −633C/T rs28954080 | 5′-UTR | F-CACGCCTGGCTGGGTTGGTT | 73.5 | CCAGAGAG[C/T]GTGCACTC | | R-TGCGGGGTTGGGGGTTCTGA | 75.9 |
| | 3 | −188C/A rs791620 | Exon-1 | F-TCGCTCCTACCAGCCACCCC | 72.7 | GCTCCTGG[A/C]GCGCCGAG | | R-GTCCTGCCTTCTGGGCACCG | 73.4 |
| | 3 | +19G/A rs2167270 | Exon-1 | F-TCGCTCCTACCAGCCACCCC | 72.7 | CAGCGCCA[A/G]CGGTTGCA | | R-GTCCTGCCTTCTGGGCACCG | 73.4 |
| | 4 | +45G/A rs145044661 | Exon-2 | F-TGAGGGGATGGTAGCCAGAGCA | 72.0 | TCAATGAC[A/G]TTTCACAC | | R-TCAGCAGGCAGAGGGGCCAA | 74.6 |
| | 5 | +102C/T rs28954113 | Exon-3 | F-GGCTCCACCCCATCCTGACCT | 72.6 | CTGGAGAA[A/C/G/T]CTCCGGGAT | | R-TGCTGGGTGGACCCCCTTCC | 74.6 |
| | 5 | +144A/G rs1800564 | Exon-3 | F-GGCTCCACCCCATCCTGACCT | 72.6 | CTTCTTCAC[A/G/T]TGCTGGCC | | R-TGCTGGGTGGACCCCCTTCC | 74.6 |
| | 5 | +328G/A rs28954114 | Exon-3 | F-GGCTCCACCCCATCCTGACCT | 72.6 | AGGACTAC[A/G]TTAAGGGA | | R-TGCTGGGTGGACCCCCTTCC | 74.6 |
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