Inhibition of Methylglyoxal-Mediated Protein Modification in Glyoxalase I Overexpressing Mouse Lenses

Figure 4

MGO-derived AGEs are inhibited by Glo I overexpression. (a) Argpyrimidine content in lenses cultured in the presence of GLD. TCA precipitated lens protein was hydrolyzed by 6 N HCl and subjected to HPLC analyses as described in the Materials and Methods. The results are the mean $\pm$ SD of 6 lenses. (${}_{}{}^{*}P<.001$). (b) HI estimation by ELISA. Microplate wells were coated overnight with 5 $\mu$g of soluble lens protein per well and incubated with a monoclonal antibody for HI followed by goat antimouse IgG. The results are the mean ± SD from 4 lenses. (${}_{}{}^{**}P<.0001$).

(a)

(b)