ERK5 Contributes to VEGF Alteration in Diabetic Retinopathy
In ECs, constitutionally active MEK5 (CAMEK5) caused increased ERK5 phosphorylation as indicated by (a) Western blot using phospho ERK5 antibody. CAMEK5 also caused mRNA upregulation of ERK5 (b), KLF2 (c) and downregulation of VEGF (d). Twenty-four hours exposure of 25 mmol/l of glucose (HG) significantly decreased VEGF mRNA expression after CAMEK5 infection (e). [mRNAs are expressed as a ratio to 18S, normalized to control, /group, : significantly different from untreated or GFP controls, †: significantly different from other groups].
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