Figure 7: Exposure to high glucose increases intracellular glucose concentration. Subconfluent cells in 24-well plate were exposed to normal glucose (NG, 5.6 mM) and high glucose (HG, 25 mM) and in the presence of 500 μM apocynin (NGA, HGA) or 1 μM MitoQ (NGMQ, HGMQ) for 4 days. The cells were washed twice with ice-cold PBS, lysed and intracellular glucose concentrations were measured using the Amplex Red glucose assay kit (Molecular Probe). Data are Means ± SEM of 5–14 separate experiments. P*<.001 versus NG; P**<.05 versus HG.