Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using a Feline Immunodeficiency Virus-Based Lentiviral Construct

Figure 5

Effect of cell differentiation on transgene GDNF expression by ELISA. Lenti-GDNF-GFP vector transduced cNPCp24 cells were seeded equally in either UM (proliferation conditions) or Ultraculture-based medium without additional growth factors but containing 10% FBS (differentiation conditions, UM-FBS). Cultures were fed 24 hours prior to collecting GDNF conditioned media for ELISA assay at which time the cells were counted. ELISA data is presented as GDNF (ng) per million cells per day in order to further evaluate whether differentiation of transduced cNPCs had an influence on transgene expression. These data are consistent with sustained GDNF overexpression, confirming the flow cytometric data (Figure 3) that showed no evidence of diminished reporter gene expression in the UM-FBS treated population of transduced cNPCs.