Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using a Feline Immunodeficiency Virus-Based Lentiviral Construct
Figure 7
GDNF expression by cNPCs before and after transduction and differentiation. Immunocytochemistry (ICC) was performed on cNPCs using a rabbit anti-human GDNF antibody to evaluate expression of GDNF at the protein level, before and after transduction and before and after exposure to growth factor deprived/FBS-containing differentiation conditions (UM-FBS). (a) Nontransduced cNPCp20 cultured in UM (proliferation conditions) exhibit baseline cytoplasmic labeling for GDNF (red). (b) Lenti-GDNF-GFP vector transduced cNPCs cultured in UM show increased intensity of GDNF (red) labeling. (c) Transduced cNPCs cultured in UM-FBS (differentiation conditions) are larger in size and show persistent overexpression of GDNF (red), that is, heterogeneously distributed among the profiles. Nuclear labeling = DAPI (blue), scale bar = 50 m.
