Journal of Ophthalmology / 2011 / Article / Tab 1 / Research Article
Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using a Feline Immunodeficiency Virus-Based Lentiviral Construct Table 1 Cat-specific primers for quantitative RT-PCR (GDNF = human).
Gene Forward primer (
-
) Reverse primer (
-
)
-actinGCCGTCTTCCCTTCCATC CTTCTCCATGTCGTCCCAGT Nestin CTGGAGCAGGAGAAGGAGAG GAAGCTGAGGGAAGCCTTG Sox2 ACCAGCTCGCAGACCTACAT TGGAGTGGGAGGAAGAGGTA Vimentin ATCCAGGAGCTACAGGCTCA GGACCTGTCTCCGGTACTCA Pax6 AGGAGGGGGAGAGAATACCA CTTTCTCGGGCAAACACATC Hes1 GCCAGCAGATATAATGGGAGA GCATCCAAAATCAGTGTTTTCA Hes5 CTCAGCCCCAAAGAGAAGAA AGGTAGCTGACGGCCATCTC Notch1 CAGTGTCTGCAGGGCTACAC CTCGCACAGAAACTCGTTGA Mash1 CATCTCCCCCAACTACTCCA CCAACATCGCTGACAAGAAA Ki-67 TCGTCTGAAGCCGGAGTTAT TCTTCTTTTCCCGATGGTTG DCX GGCTGACCTGACTCGATCTC GCTTTCATATTGGCGGATGT
3-tubulinCATTCTCGTGGACCTTGAGC GCAGTCGCAATTCTCACATT Map2 ACCTAAGCCATGTGACATCCA CTCCAGGTACATGGTGAGCA PKC-alpha TTCACAAGAGGTGCCATGAA CCATACAGCAATGACCCACA GFAP CGGTTTTTGAGGAAGATCCA TTGGACCGATACCACTCCTC Lhx2 GATCTGGCGGCCTACAAC AGGACCCGTTTGGTGAGG CD81 CCACAGACCACCAACCTTCT CAGGCACTGGGACTCCTG CD133 AGGAAGTGCTTTGCGGTCT TGCCAGTTTCCGAGTCTTTT NCAM (CD56) AGAACAAGGCTGGAGAGCAG TTTCGGGTAGAAGTCCTCCA EGFR AACTGTGAGGTGGTCCTTGG CGCAGTCCGGTTTTATTTGT NagoA TTTGCAGTGTTGATGTGGGTA TAACAGGAACGCTGAAGAGTGA SDF1 ACAGATGTCCTTGCCGATTC CCACTTCAATTTCGGGTCAA CXCR4 TCTGTGGCAGACCTCCTCTT TTTCAGCCAACAGCTTCCTT Cyclin D2 CAAGATCACCAACACGGATG ATATCCCGCACGTCTGTAGG Pbx1 CTCCGATTACAGAGCCAAGC GCTGACCATACGCTCGATCT FABP7 TGGAGGCTTTCTGTGCTACC TGCTTTGTGTCCTGATCACC AQP4 TACACTGGTGCCAGCATGA CACCAGCGAGGACAGCTC Nucleostemin CAGTGGTGTTCAGAGCCTCA CCGAATGGCTTTGCTGTAA Synapsin1 ACGACGTACCCTGTGGTTGT CGTCATATTTGGCGTCAATG Caspase 3 ATGGAGAACAGTGAAAACTCAGTGG AATTATTATACATAAACCCATTTCAGG Bax 4 CTGAGCAGATCATGAAGACAGG GTCCAGTTCATCTCCGATGC hGDNF* TGGGCTATGAAACCAAGGAG CAACATGCCTGCCCTACTTT
*Human GDNF gene.
