Schematic representation of the mating scheme of dominant (G1) or recessive (G3) screens. Male mice were mutagenized (3 weekly doses, 80 mg/kg) and mated to WT females after 4 weeks. If any female was pregnant within 5 weeks, the mating was discarded. If, however, male mice impregnated a female after that, the resulting G1 males were crossed to their respective female counterparts, and the G2 progeny were backcrossed to the G1 fathers to generate G3 offspring.
