Review Article
Multiphoton Microscopy for Ophthalmic Imaging
Table 1
Optimal imaging contrast mechanism for different biological molecules.
| | Compound | Imaging technique (excitation/emission wavelengths) |
| | NAD(P)H | 2PAF/FLIM (excitation 700–730 nm/emission peak 460 nm) [16] |
| | FAD | 2PAF/FLIM (excitation 700–900 nm/emission peak 525 nm) [16] |
| | Elastin | 2PAF (excitation 700–740 nm/emission peak 400 nm) [15, 19] |
| | Collagen | SHG (SHG excitation is tunable/emission at one-half the excitation wavelength) [15] |
| | Lipids | THG/CARS (THG excitation is tunable with emission at one-third the excitation wavelength) [14] |
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