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Journal of Ophthalmology
Volume 2012, Article ID 303459, 6 pages
Research Article

Advanced Corneal Cross-Linking System with Fluorescence Dosimetry

Research and Development, Avedro Inc., Waltham, MA 02451, USA

Received 3 February 2012; Revised 27 April 2012; Accepted 7 May 2012

Academic Editor: David A. Wilkie

Copyright © 2012 Marc D. Friedman et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. This paper describes an advanced system that combines corneal cross-linking with riboflavin with fluorescence dosimetry, the ability to measure riboflavin diffusion within the cornea both before and during UVA treatment. Methods and Results. A corneal cross-linking system utilizing a digital micromirror device (DMD) was assembled and used to measure diffusion coefficients of 0.1% riboflavin in 20% dextran in porcine eyes. A value of ( 3 . 3 ± 0 . 2 ) × 1 0 7  cm2/s was obtained for the stroma. Diffusion coefficients for the transepithelial formulation of 0.1% riboflavin in 0.44% saline and 0.02% BAK were also measured to be 4 . 7 ± 0 . 3 × 1 0 8  cm2/s for epithelium only and ( 4 . 6 ± 0 . 4 ) × 1 0 7  cm2/s for stroma only. Riboflavin consumption during a UVA treatment was also demonstrated. Conclusion. A new advanced corneal cross-linking system with fluorescence dosimetry of riboflavin has been demonstrated. It is hoped that this method may play a significant role in determining the underlying mechanisms of corneal cross-linking and assist with the development of additional riboflavin formulations. Moreover, dosimetry may prove valuable in providing a method to account for the biological differences between individuals, potentially informing cornea-specific UVA treatment doses in real time.