Clinical Study

Effects of Fluorescein Staining on Laser In Vivo Confocal Microscopy Images of the Cornea

Table 1

Percentage of respondents who were correct or incorrect in identifying the presence or absence of fluorescein in confocal microscopy images of the cornea. Results are mean ± standard deviation for results from 21 to 25 respondents per image (up to 4 respondents abstained from responding to various images).

SamplePercentage of respondents correct per image, %Percentage of respondents incorrect per image, %

Epithelium images
 No fluorescein, 𝑛 = 1 9 images 4 4 ± 1 7 5 6 ± 1 7
 With fluorescein, 𝑛 = 1 5 images 5 3 ± 1 2 4 7 ± 1 2
Overall, epithelium, 𝑛 = 3 4 images 48 ± 14 52 ± 15
Subbasal nerve plexus images
 No fluorescein, 𝑛 = 1 0 images 4 8 ± 1 4 5 2 ± 1 4
 With fluorescein, 𝑛 = 1 9 images 5 0 ± 1 0 5 0 ± 1 0
Overall, nerves, 𝑛 = 2 9 images 49 ± 1151 ± 11
Stroma images
 No fluorescein, 𝑛 = 1 5 images 6 0 ± 1 8 4 0 ± 1 8
 With fluorescein, 𝑛 = 2 1 images 3 6 ± 2 0 6 4 ± 2 0
Overall, stroma, 𝑛 = 3 6 images 46 ± 2354 ± 23

All 3 corneal layers, 𝑛 = 9 9 images 4 8 ± 1 7 5 2 ± 1 7