Mouse cornea lymphangiogenesis model. No lymphatic vessels exist in the normal mammalian cornea. Implantation of growth factor or cytokine into a surgically created micropocket in the mouse cornea stroma (a) or corneal injury by suture (b) induces lymphangiogenic response. Corneal lymphangiogenesis as well as angiogenesis can be examined 6 or 7 days after pellet implantation or suture, respectively (a) and (b). Double staining of corneal flat mounts for lymphangiogenic (LYVE-1, red) endothelium and angiogenic (CD31, green) with immunohistochemistry (c).
