Optimization of Storage Temperature for Cultured ARPE-19 Cells
Figure 2
A calcein-acetoxymethyl ester (CAM) reagent, which exclusively stains living cells, was used to analyze cell survival. To validate the method, control cells and methanol-fixed cells were incubated with PBS containing 1 M CAM (green). (a) Control cells were CAM+ (green). (b) Corresponding phase contrast micrograph to (a). (c) Fixed cells were CAM−. (d) Corresponding phase contrast micrograph to (c). (e) Cells were seeded in multidishes in increasing concentrations and incubated for two hours to ensure attachment to the substrate. The CAM reagent was added to the cells for one hour, and the CAM fluorescence was measured with a microplate fluorometer. The number of seeded cells correlated significantly with the measured CAM fluorescence, thereby proving great accuracy of the microplate reader measurements.
