Research Article

Optimization of Storage Temperature for Cultured ARPE-19 Cells

Figure 3

Positive control to the caspase-3 antibody. Cultured RPE cells were incubated with 1  M staurosporine for 24 hours in order to trigger expression of caspase-3 and induce cell apoptosis. (a) Photomicrograph showing immunostaining of caspase-3 (red) in apoptotic cells treated with staurosporine. Nuclei were stained with DAPI (blue). Original magnification: ×200. (b) Negative control showing only DAPI staining (blue) when no staurosporine is added. Original magnification: ×200.
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216359.fig.003b
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