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Journal of Ophthalmology
Volume 2013, Article ID 670947, 5 pages
http://dx.doi.org/10.1155/2013/670947
Research Article

Evaluation of the HB&L System for the Microbiological Screening of Storage Medium for Organ-Cultured Corneas

1The Veneto Eye Bank Foundation, Via Paccagnella 11, 30174 Venice, Italy
2Unità Operativa Complessa Laboratorio Analisi Chimico-Cliniche e Microbiologiche, Ospedale dell’ Angelo, 30174 Venezia, Italy
3Università degli Studi di Padova, Dipartimento di Scienze Cardiologiche, Toraciche e Vascolari, Via Giustiniani, 2-35128 Padova, Italy

Received 24 June 2013; Accepted 30 July 2013

Academic Editor: Shivalingappa K. Swamynathan

Copyright © 2013 D. Camposampiero et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aims. To compare HB&L and BACTEC systems for detecting the microorganisms contaminating the corneal storage liquid preserved at 31°C. Methods. Human donor corneas were stored at 4°C followed by preservation at 31°C. Samples of the storage medium were inoculated in BACTEC Peds Plus/F (aerobic microorganisms), BACTEC Plus Anaerobic/F (anaerobic microorganisms), and HB&L bottles. The tests were performed (a) after six days of storage, (b) end of storage, and (c) after 24 hours of preservation in deturgescent liquid sequentially. 10,655 storage and deturgescent media samples were subjected to microbiological control using BACTEC (6-day incubation) and HB&L (24-hour incubation) systems simultaneously. BACTEC positive/negative refers to both/either aerobic and anaerobic positives/negatives, whereas HB&L can only detect the aerobic microbes, and therefore the positives/negatives depend on the presence/absence of aerobic microorganisms. Results. 147 (1.38%) samples were identified positive with at least one of the two methods. 127 samples (134 identified microorganisms) were positive with both HB&L and BACTEC. 14 HB&L+/BACTEC− and 6 BACTEC+/HB&L− were identified. Sensitivity (95.5%), specificity (99.8%), and positive (90.1%) and negative predictive values (99.9%) were high with HB&L considering a 3.5% annual contamination rate. Conclusion. HB&L is a rapid system for detecting microorganisms in corneal storage medium in addition to the existing methods.