Cell Models to Study Regulation of Cell Transformation in Pathologies of Retinal Pigment Epithelium
Some characteristics of adult human RPE cells in vitro. (a) The results of real-time PCR analysis of TUBB3 expression in primary culture (PC) and subsequent passages (P1–P4) of RPE cells in adhesive monolayers, compared to freshly isolated RPE (RPE Tissue). (b) Immunoperoxidase staining for TUBB3 (brown) in passage 2 RPE cells. (c) Immunoperoxidase staining (brown) for neurofilaments 68 and 200 in passage 4 RPE cells. Cell nuclei are stained with hematoxylin. Scale bar, 60 μm.