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Journal of Ophthalmology
Volume 2015, Article ID 259123, 14 pages
Research Article

Thickness Mapping of Eleven Retinal Layers Segmented Using the Diffusion Maps Method in Normal Eyes

1Biomedical Engineering Department, Medical Image & Signal Processing Research Center, Isfahan University of Medical Sciences, Isfahan 81745, Iran
2Faculty of Engineering and Natural Sciences, Sabanci University, 34956 Istanbul, Turkey
3The Iowa Institute for Biomedical Imaging, The University of Iowa, Iowa City, IA 52242, USA
4Noor Ophthalmology Research Center, Tehran 1968653111, Iran
5Department of Ophthalmology & Visual Science, The University of Iowa, Iowa City, IA 52242, USA
6VA Medical Center, Iowa City, IA 52246, USA
7Department of Electrical & Computer Engineering, The University of Iowa, Iowa City, IA 52242, USA

Received 29 October 2014; Revised 22 February 2015; Accepted 10 March 2015

Academic Editor: Bartosz Sikorski

Copyright © 2015 Raheleh Kafieh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study was conducted to determine the thickness map of eleven retinal layers in normal subjects by spectral domain optical coherence tomography (SD-OCT) and evaluate their association with sex and age. Mean regional retinal thickness of 11 retinal layers was obtained by automatic three-dimensional diffusion map based method in 112 normal eyes of 76 Iranian subjects. We applied our previously reported 3D intraretinal fast layer segmentation which does not require edge-based image information but rather relies on regional image texture. The thickness maps are compared among 9 macular sectors within 3 concentric circles as defined by ETDRS. The thickness map of central foveal area in layers 1, 3, and 4 displayed the minimum thickness. Maximum thickness was observed in nasal to the fovea of layer 1 and in a circular pattern in the parafoveal retinal area of layers 2, 3, and 4 and in central foveal area of layer 6. Temporal and inferior quadrants of the total retinal thickness and most of other quadrants of layer 1 were significantly greater in the men than in the women. Surrounding eight sectors of total retinal thickness and a limited number of sectors in layers 1 and 4 significantly correlated with age.