Research Article

Effect of Storage Temperature on Key Functions of Cultured Retinal Pigment Epithelial Cells

Table 1

Expression of genes involved in key functions of the RPE at different temperatures compared to controls.

Gene symbolGene descriptionFold change
4°C versus C16°C versus C37°C versus C

Phagocytosis
ANXA2Annexin A2−1.07−1.06−1.21
ARPC2Actin related protein 2/3 complex, subunit 2, 34 kDa−1.04−1.01−1.10
CD81CD81 molecule−1.041.01−1.10
CTSDCathepsin D1.111.05 1.23
GAS6Growth arrest-specific 6−1.03−1.01−1.09
ITGAVIntegrin, alpha V1.02−1.05−1.05
ITGB2Integrin, beta 21.061.031.06
ITGB5Integrin, beta 51.01−1.011.01
LAMP2Lysosomal-associated membrane protein 21.03−1.001.13
MerTKMER protooncogene, tyrosine kinase1.041.001.73
MFGE8Milk fat globule-EGF factor 8 protein1.07−1.011.32
MYO7AMyosin VIIa1.311.021.28
PROS1Protein S1.061.021.73
PTDSS1Phosphatidylserine synthase 1−1.151.11−1.18
TLR4Toll-like receptor 4−1.07−1.08−1.15
WASPWiskott-Aldrich syndrome1.061.101.23

Pigment synthesis
GPR143G protein-coupled receptor 1431.051.141.33
MITFMicrophthalmia-associated transcription factor1.12−2.351.03
OCA2Oculocutaneous albinism II1.031.051.03
PMELPremelanosome protein1.121.03−1.02
SLC45A2Solute carrier family 45, member 21.181.371.28
TYRP1Tyrosinase-related protein 11.031.023.62

Visual cycle
CRABP1Cellular retinoic acid binding protein 11.081.141.11
CRABP2Cellular retinoic acid binding protein 2−1.031.77−1.40
LRATLecithin retinol acyltransferase−1.01−1.21−1.23
RBP1Retinol binding protein 1, cellular−1.09−1.001.15
RBP3Retinol binding protein 3, interstitial1.051.061.03
RBP5Retinol binding protein 5, cellular1.131.031.14
RBP7Retinol binding protein 7, cellular1.011.04−1.60
RDH11Retinol dehydrogenase 11−1.191.001.06
RDH14Retinol dehydrogenase 141.03−1.091.15
RLBP1Retinaldehyde binding protein 1−1.001.21−1.31

Adherens junctions
CDH1Cadherin 1, type 1, E-cadherin (epithelial)1.111.221.21
CDH2Cadherin 2, type 1, N-cadherin (neuronal)−1.02−1.07−1.23
CDH4Cadherin 4, type 1, R-cadherin (retinal)−1.021.07−1.35
CDH6Cadherin 6, type 2, K-cadherin (fetal kidney)1.12−1.12−3.04
CDH10Cadherin 10, type 2 (T2-cadherin)1.06−1.27−1.73
CDH11Cadherin 11, type 2, OB-cadherin (osteoblast)−1.08−1.43−2.16
CDH13Cadherin 13−1.051.032.02
CDH15Cadherin 151.081.121.05
CDH16Cadherin 16, KSP-cadherin1.081.091.07
CDH17Cadherin 17, LI cadherin (liver-intestine)1.14−1.021.35
CDH22Cadherin 22, type 21.061.021.04
CDH23Cadherin-related 231.091.141.08
CDH24Cadherin 24, type 21.011.011.13
DSC1Desmocollin 11.111.088.34
DSC2Desmocollin 21.061.011.11
DSC3Desmocollin 31.09−1.04−1.17

Tight junctions
ACTBActin, beta−1.05−1.02−1.18
CALM1Calmodulin 1 (phosphorylase kinase, delta)−1.041.09−1.18
CLDN3Claudin 31.14−1.01−1.16
CLDN9Claudin 9−1.021.081.21
CLDN11Claudin 11−1.111.07−2.91
CLDN15Claudin 151.04−1.071.28
CLDN18Claudin 181.121.221.12
CLDN19Claudin 191.131.121.15
CLDN23Claudin 231.131.181.14
CRB3Crumbs family member 31.171.171.15
CTNNACatenin (cadherin-associated protein), alpha 1−1.03−1.031.03
F11RF11 receptor−1.021.04−1.52
JAM3Junctional adhesion molecule 3−1.081.01−1.18
MAGI1Membrane associated guanylate kinase, WW and PDZ domain containing 11.03−1.50−1.08
MAGI3Membrane associated guanylate kinase, WW and PDZ domain containing 3−1.04−1.82−1.33
MPDZMultiple PDZ domain protein1.09−1.291.01
MYO7AMyosin VIIA1.311.021.28
OCLNOccludin1.09−1.151.49
PTENPhosphatase and tensin homolog1.02−1.01−1.14
RAB3BRAB3B, member RAS oncogene family−1.151.08−2.19
TJP1 Tight junction protein 11.02−1.10−1.02
TJP2Tight junction protein 2−1.011.25−1.40
TJP3Tight junction protein 31.061.081.04

Glucose transport
SLC2A1Solute carrier family 2 (facilitated glucose transporter), member 11.04−1.011.05
SLC2A3Solute carrier family 2 (facilitated glucose transporter), member 31.112.001.13
SLC2A4Solute carrier family 2 (facilitated glucose transporter), member 41.051.121.05
SLC2A5Solute carrier family 2 (facilitated glucose transporter), member 51.101.161.13
SLC2A6Solute carrier family 2 (facilitated glucose transporter), member 6−1.111.03−1.06
SLC2A8Solute carrier family 2 (facilitated glucose transporter), member 81.011.211.21
SLC2A10Solute carrier family 2 (facilitated glucose transporter), member 101.071.131.14
SLC2A11Solute carrier family 2 (facilitated glucose transporter), member 111.08−1.011.07
SLC2A12Solute carrier family 2 (facilitated glucose transporter), member 12−1.08−1.39−2.69
SLC2A13Solute carrier family 2 (facilitated glucose transporter), member 131.01−1.05−1.08
SLC2A14Solute carrier family 2 (facilitated glucose transporter), member 141.081.581.07

Na-K-ATPase
ATP1A1ATPase, Na+/K+ transporting, alpha 1 polypeptide−1.04−1.031.21
ATP1A2ATPase, Na+/K+ transporting, alpha 2 polypeptide1.081.061.22
ATP1A3ATPase, Na+/K+ transporting, alpha 3 polypeptide−1.011.381.17
ATP1B1ATPase, Na+/K+ transporting, beta 1 polypeptide1.011.12−1.01
ATP1B2ATPase, Na+/K+ transporting, beta 2 polypeptide1.101.171.09
ATP1B3ATPase, Na+/K+ transporting, beta 3 polypeptide−1.161.101.49

values < 0.05 are marked in bold font.