Risk Factors in Normal-Tension Glaucoma and High-Tension Glaucoma in relation to Polymorphisms of Endothelin-1 Gene and Endothelin-1 Receptor Type A Gene
Table 2
Reaction mixture composition.
Ingredient
Volume in L/well
TaqMan Universal PCR Master Mix
12.5
Stock of SNP
1.25
Isolated patient’s DNA (10 ng)
11.25
Total
25
Buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, DNase-free water
