The Polymorphisms with Cataract Susceptibility Impair the EPHA2 Receptor Stability and Its Cytoprotective Function

Figure 1

The evolutional conservation of cataract-associated SNP and the overexpression of EPHA2 in lens epithelial cells. (a) The protein sequences of human, macaque, rat, and mouse EPHA2 were aligned for multiple comparison. The functional polymorphisms rs1085371 and rs2291806 are highlighted here to show the missense mutation in EPHA2. The sequence alignment shows the evolutional conservation of both amino acids with substitution. (b) The human lens epithelial cells (HLECs) were infected with lentivirus encoding wild-type EPHA2, , or . The images captured with fluorescent microscopy 72 h after infection are shown here. Scale bar: 50 μm. (c) The infection efficiency was quantified by counting GFP-positive cells and total number of cells. Data are shown as the mean ± SEM.

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