The Polymorphisms with Cataract Susceptibility Impair the EPHA2 Receptor Stability and Its Cytoprotective Function

Figure 2

The protein destabilization caused by cataract-associated missense mutation. (a) The EPHA2 mRNA transcriptional level was measured with real-time PCR after HLECs were overexpressed with wild-type or mutant EPHA2 for 48 h. The EPHA2 mRNA level was normalized with the internal control β-actin and the data are presented as ratio of vector control. (b) The EPHA2 protein expression was tested with western blotting after the lentivirus-mediated overexpression in HLECs. (c) Quantitative analysis shows the impaired protein stability of EPHA2 with missense mutation. The EPHA2 protein level was normalized with the internal control GAPDH. Data are shown as the mean ± SEM. , versus vector control; , versus wild-type EPHA2.

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