The Polymorphisms with Cataract Susceptibility Impair the EPHA2 Receptor Stability and Its Cytoprotective Function

Figure 3

The functional polymorphisms abolish EPHA2-mediated cytoprotective effect. (a) The experimental paradigm. The HLECs were seeded and infected with lentivirus expressing wild-type EPHA2, , or . The infected cells were treated with 200 μM H₂O₂ to mimic the light-induced oxidative stress in lens. (b-c) The cell viability was assayed before and after H₂O₂ treatment following lentivirus-based gene overexpression. The quantification reveals the cytoprotective effect of wild-type EPHA2 and the loss of function in cataract-associated mutants. Data are shown as the mean ± SEM. , versus vector control; , versus wild-type EPHA2.

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