The Polymorphisms with Cataract Susceptibility Impair the EPHA2 Receptor Stability and Its Cytoprotective Function

Figure 4

Compromised antiapoptotic function in cataract-associated genetic mutants. (a) After lentiviral infection to introduce control vector, EPHA2, , or into the cells and the administration with 200 μM H₂O₂, the HLECs were digested, dissociated, and stained with either APC-annexin V or 7-AAD, followed by the fluorescence activated cell sorting to analyze the early and late apoptosis. The GFP signal intensity was compromised if the HLECs undergo oxidative stress-induced apoptosis. (b-c) The statistical analysis shows that overexpression of wild-type EPHA2 reduces the proportion of both early and late apoptotic cells. The missense mutations abolish the antiapoptotic effect against oxidative damage. , , and versus vector control; , versus wild-type EPHA2.

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