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Journal of Ophthalmology
Volume 2015 (2015), Article ID 612728, 6 pages
Research Article

Responses of the Ocular Anterior Segment and Refraction to 0.5% Tropicamide in Chinese School-Aged Children of Myopia, Emmetropia, and Hyperopia

1Shanghai Eye Disease Prevention & Treatment Center, Shanghai 200040, China
2Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China
3Chinese Medicine Hospital of Zhejiang Province, Hangzhou, Zhejiang 310006, China

Received 4 June 2015; Revised 17 August 2015; Accepted 18 August 2015

Academic Editor: Terri L. Young

Copyright © 2015 Ying Yuan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. To investigate the changes of anterior segment after cycloplegia and estimate the association of such changes with the changes of refraction in Chinese school-aged children of myopia, emmetropia, and hyperopia. Methods. 309 children were recruited and eligible subjects were assigned to three groups: hyperopia, emmetropia, or myopia. Cycloplegia was achieved with five cycles of 0.5% tropicamide. The Pentacam system was used to measure the parameters of interest before and after cycloplegia. Results. In the myopic group, the lenses were thinner and the lens position was significantly more posterior than that of the emmetropic and hyperopic groups in the cycloplegic status. The correlations between refraction and lens thickness (age adjusted; , ), and lens position (age adjusted; , ) were found. After cycloplegia, ACD and ACV significantly increased, while ACA significantly decreased. Changes in refraction, ACD, ACV, and ACA were significantly different among the three groups (, all). Changes of refraction were correlated with changes of ACD (, ). Conclusions. Myopia presented thinner lenses and smaller changes of anterior segment and refraction after cycloplegia when compared to emmetropia and hyperopia. Changes of anterior chamber depth were correlated with refraction changes. This may contribute to a better understanding of the relationship between anterior segment and myopia.

1. Introduction

Myopia is a significant public health problem worldwide and is especially prominent in East Asian countries such as China and Singapore [16]. The incidence of myopia tends to increase in adolescence and is the main cause of low vision among the population [7]. The exact mechanism underlying development of myopia has not been well elucidated, but it is clear that the ocular refraction components including corneal, anterior segment, lens thickness (LT), vitreous chamber depth, and axial length are mismatched in myopia. The crystalline lens accounts for almost 20% of the eye’s refracting power. During emmetropization of human eyes, the crystalline lens undergoes thinning and flattening [8, 9]. However, the role of the lens in myopia onset and progression is still controversial. A longitudinal study from Singapore showed that changes in LT were biphasic in different refractive status [10]. Other studies with myopia models indicated that the role of the lens in refractive error development was nonexistent [11]. Moreover, the importance of lens position (LP), defined as anterior chamber depth (ACD) + LT/2, has rarely been investigated in myopes. Therefore, we conducted this cross-sectional study to investigate the association between LT, LP, and myopia.

Evaluation of the ocular anterior segment was essential in clinical ophthalmology. During accommodation, the LT and anterior lens curvature changed which would lead to a change in anterior segment structure. The role of anterior segment in myopia development has also come to the attention of researchers currently. Previous work reported that the anterior chamber angle (ACA) and ACD increased with myopia progression [12, 13]. Meanwhile, myopes usually showed insufficient accommodation or even accommodative lag [14, 15]. However, the effect of accommodation on the anterior segment of myopia remained unclear. So we investigated the relationship between change of anterior segment and change of refraction before and after cycloplegia here.

In this study, 0.5% tropicamide was used to relax accommodation and Pentacam was used to investigate the front corneal radius, back corneal radius, ACD, ACV, and ACA in myopia, emmetropia, and hyperopia before and after cycloplegia. Subjective refraction was also measured before and after application of tropicamide. Additionally, LT and LP after cycloplegia were measured to investigate the role of lens in myopia development. The results would shed more light on the relationship between anterior segment and myopia.

2. Materials and Methods

2.1. Subjects

Three hundred and nine children from one primary school in Chongming, Shanghai, China, were invited to participate in our study. Children with eye diseases and a history of ocular trauma and surgery or who could not cooperate during Pentacam measurements were excluded. The parameters of interest were measured bilaterally but only data from the right eyes were compared. This study was approved by the Human Research Committee of Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine. We obtained informed written consent from at least one legal guardian, as well as verbal consent, from each child. The study’s protocol conformed to the restrictions established by the Declaration of Helsinki.

2.2. Ocular Examination

All participants underwent a complete ophthalmic examination which included visual acuity, slit-lamp biomicroscopy, and the refraction of noncycloplegic and cycloplegic state. Best-corrected visual acuity was assessed monocularly with linear logMAR charts if the child had an uncorrected visual acuity higher than (i.e., vision > ). A Haag-Streit slit-lamp (Koeniz, Switzerland) was used to examine the anterior segment. Five cycles of tropicamide (0.5%, one drop per cycle) were administered 5 minutes apart. About 30 minutes after application of the eye drops, children without pupillary light reflex were examined by an autorefractometer (RK-F1, Canon), which generated 5 valid readings of refraction, and the median value was used for analyses.

2.3. Pentacam Measurement

Anterior segment parameters were measured before and after application of tropicamide (determined by an absence of pupillary light reflex) with the Pentacam system. The Oculus Pentacam is a noninvasive evaluative test of ocular anterior segment topography using Scheimpflug photography. The mechanics of the system have been described in detail elsewhere [16]. Participants were seated comfortably and asked to keep their chin on the chinrest and forehead resting against the forehead strap. Every participant was instructed to keep both eyes open and to fixate on the black target in the center of the blue fixation beam when the instrument was in scan mode. The real-time image on the computer screen was adjusted manually by moving a joystick in the directions indicated on the screen. When the image met -, -, and -plane alignment criteria, the scan was automatically commenced in an automatic release mode. Only scans registering as acceptable (‘‘OK’’) on the instrument’s “QS” (quality specification) output were used for analysis.

2.4. Definitions and Statistical Analysis

Spherical equivalence (SE) was calculated as the spherical power plus half-negative cylinder power. Emmetropia was defined as an SE between −0.5 D and +0.5 D after cycloplegia, myopia as an SE ≤ −0.5 D, and hyperopia as an SE ≥ +0.5 D. The results were expressed as mean ± standard deviation. All statistical evaluations were performed using SAS version 9.2 (SAS Institute). Shapiro-Wilk test was used to assess normal distribution. Levene’s test was used to test the variance homogeneity. Paired -test, independent sample -test, and one-way analysis of variance (ANOVA) with pairwise Bonferroni posttests were used to analyze the differences between variables. Wilcoxon signed ranks test and Kruskal-Wallis test were applied when the normality assumption and homogeneity of the variance assumption were violated. Pearson correlation coefficient was used to determine the association between refractive error and biometric parameters. was considered statistically significant. All tests were two-tailed.

3. Results

Of the 309 participants examined, 281 children (90.94% of participants) aged 6–13 years (mean, 8.87 ± 1.54 years) were selected for analysis in this study, including 138 girls (49.11%) and 143 boys (50.89%) (Table 1). Twenty-eight children (9.96%) were excluded due to poor cooperation or low scan quality. No subject had a pupil response to light after the application of 5 drops of 0.5% tropicamide. All measurements of the anterior segment were normally distributed both before and after cycloplegia. The overall distributions of measurements before and after cycloplegia, as well changes between the two sessions, are detailed in Table 2.

Table 1: Patient demographics and refractive status.
Table 2: Anterior segment parameters before and after cycloplegia among refractive groups.
3.1. Relationship between Crystalline Lens and Refractive Status

The mean LT and LP in the cycloplegic state were significantly different among the myopic, emmetropic, and hyperopic groups (). A positive association was found between the LT and refractive status (age adjusted; , ; Figure 1), while a negative association was noted between the LP and refractive error (age adjusted; , ; Figure 2). The LT significantly decreased with increasing age, from age 6 to 13 years (SE adjusted; , ). No significant difference was found in LT and LP between boys and girls. And there was no significant difference in sex among the myopic, emmetropic, and hyperopic groups. We analyzed the difference in age among the three groups and found the age in the myopic group was different from the emmetropic and hyperopic groups.

Figure 1: Association between lens thickness and refractive status in the cycloplegia.
Figure 2: Association between lens position and refractive status in the cycloplegia.
3.2. Tropicamide-Induced Changes in Anterior Segment Parameters and Their Association with Refractive Status

After application of tropicamide, the values of ACD and ACV increased (both ), while ACA decreased significantly (). The front and back corneal radius and corneal astigmatism were not significantly different from before cycloplegia (, resp.). A high correlation was found between ACD and ACV both before and after cycloplegia (, , and , , resp.).

Changes in the ACD (ΔACD) and volume (ΔACV) after cycloplegia were less pronounced in the myopes than those in the emmetropes or hyperopes (; Table 2). The change of ACA (ΔACA) was different among refractive groups (; Table 2). ΔACA of the myopic and emmetropic group had a decrement (, , resp.), while that of the hyperopic group had an increment (). There was no significant difference between genders for ΔACD, ΔACV, and ΔACA in any group (Table 3). No significant difference based on age was found for corneal radius, corneal astigmatism, ACD, ACV, and ACA.

Table 3: Difference in anterior segment parameters between genders among the three groups.
3.3. Relationship between Changes in SE (ΔSE) and Changes in Anterior Segment

Cycloplegia with application of 0.5% tropicamide significantly changed the mean SE from −0.87 D to −0.54 D (; Table 1), which was statistically smaller in the myopic group (from −1.93 D to −1.85 D, ΔSE 0.08 ± 0.48 D) than in the emmetropic (from −0.30 D to 0.03 D, ΔSE 0.33 ± 0.53 D) and hyperopic (from −0.30 D to 1.16 D, ΔSE 0.86 ± 0.77 D) groups. However, changes of ACD per diopter in any group were not statistically significant, with myopes presenting 0.16 ± 0.33 mm/D, emmetropes 0.21 ± 0.45 mm/D, and hyperopes 0.20 ± 0.62 mm/D. The association between ΔACD, ΔACV, and ΔSE was demonstrated by Pearson correlation. It was found that ΔSE was positively associated with ΔACD (, ; Figure 3). The weaker association was found between ΔSE and ΔACV (, ).

Figure 3: Association between the change of anterior chamber depth and the change of refraction.

4. Discussion

In the current study, we evaluated the change of anterior segment and refraction and their associations due to cycloplegia in different refractive status. The finding indicated that ACD, ACV, and ACA changed significantly after cycloplegia. And ΔACD was correlated with refraction changes. Myopic children had smaller ΔACD, ΔACV, and ΔACA when compared to emmetropic and hyperopic children.

We found that lenses in the myopic group were significantly thinner than that in the emmetropic and hyperopic group, with LT 3.31 mm in myopes, 3.43 mm in emmetropes, and 3.46 mm in hyperopes. In human eyes, every diopter of reduced accommodation produces only a 0.06 mm decrease in lens thickness [17]. The 0.12 mm decreased in the LT in the myopia would result in an excess −2 D. This was consistent with the average refractive error −1.85 ± 1.25 D in the myopic group. We further took advantage of the Emsley simplified eye model and relevant geometrical optics mechanism to probe the changes of LT as well as LP and their effect on the development of myopia. For eyes with thinner lenses, the focal length would increase due to the reduction lens refractive power. Additionally, the nodal point would have backward movement as LP changed. Both of them could lead to the image of objects located posteriorly to the retina. This can result in hyperopic defocus of retina and then increase the degree of myopia.

Animal experiments have proved that a concave lens could lead to myopia onset and development [18], which was consistent with the theory of hyperopic defocus. Lambert has found that removing the crystalline lens and implanting an intraocular lens in a neonatal monkey eye retarded its axial elongation [19]. This phenomenon may be caused by the relative static intraocular lens which cannot become thinner with age. Gradually, these eyes with such intraocular lenses produced myopic defocus. A longitudinal study in Chinese children revealed that newly developed myopia had slightly lower lens power than in children with persistent emmetropia, when they were still at emmetropic state [20]. Consequently, the crystalline lens could be an important contributor to myopia onset and development and may be a clue to the physician to observe refractive development. However, the children in our study, aged 6 to 13 years, had mild to moderate myopia. Further studies need to be conducted to investigate the role of the crystalline lens in myopia progression, including different degrees of myopia.

We found a significant increase in ACD as well as ACV after application of tropicamide in any group, which was consistent with the result of previous reports [2123]. However, there was no significant difference in the front and back corneal radii before and after cycloplegia. In sum, the changes in ACD and ACV may be due to the decrease of LT which was caused by axial flattening and backward movement of the lens. Furthermore, a smaller increment in ACD was seen in the myopic group and a greater increment in the emmetropic and hyperopic groups in our study. It may be one of the optical structure basements to insufficient accommodation in myopia. When accommodation occurs, the refractive index of ocular increased due to the increase of LT and decrease of anterior segment. In myopia, it had a smaller change in anterior segment, which may lead to a less increased refractive power during accommodation.

In addition, after relaxing accommodation by tropicamide, the change of refraction was smaller in myopia compared to emmetropia and hyperopia. It was thought that the refraction change after cycloplegia was mainly associated with the decrease of lens power which was due to the decrease of the lens surfaces curvature, lens position, and lens thickness [24]. However, the accompanying changes in anterior chamber depth may also play a role in the refraction change after cycloplegia. In this study, we found a significant change of anterior segment after cycloplegia and found a positive correlation between ΔACD and ΔSE. The probability was significant, although the correlation coefficient was low. ΔACD caused by per diopter change in myopia is slightly smaller. It may prove that the insufficient accommodation in myopia is related to the anterior structure. This may be caused by the thickened ciliary muscle in myopia which had poor contractility and dilatability [25]. As the subjects had mostly mild to moderate myopia in our study, the function of ciliary muscle may not change significantly. A 0.19 mm ΔACD per diopter accommodation was found in our study. This was different from the result of previous studies, which measured the ocular dimensions at different accommodation stimuli [26]. It may be due to the fact that the actual accommodative responses were less than the stimuli especially in myopia.

We also found that ACA changed after cycloplegia and ΔACA differed in different refractive groups. ΔACA in the hyperopic group was a slight increment, whereas in the emmetropic and myopic groups there was a decrement (although the change in the myopic group was statistically insignificant). Contrary to our results, Tsai and associates [27] recently found that the ACA in myopes and emmetropes increased significantly after diagnostic cycloplegia using 1% tropicamide, as observed with the Pentacam system. The discrepancy between their results and ours can be explained by the following. Firstly, the concentrations of tropicamide were different (0.5% in our study and 1% in Tsai et al.’s study), which could cause cycloplegia and pupil dilation to different degrees and result in different changes in anterior lens displacement. Secondly, the baseline anterior segment parameters were different. Subjects in the Tsai et al. study had larger ACA before cycloplegia than ours. Therefore, the value of ΔACA may depend on both the anterior segment configurations at baseline and the response to tropicamide. However, the exact mechanism needs further investigation. Tsai et al. [27] also proved that there was no association between the change of intraocular pressure (IOP) and the change of ACA in school-aged children. So the importance of the change of ACA after cycloplegia in youths is different from that in elders. The relationships between ACA and refractive status and IOP are still not clear.

There were some limitations in this study. First, the pharmacological cycloplegia after application of 0.5% tropicamide may be incomplete. It was thought that good cycloplegia and dilation were achieved with atropine. But it took too long to reach maximum dilation and had more adverse effect [28]. In China, 0.5% tropicamide has been routinely used for refraction or dilated fundus examination in children. For this, the result of our study may be more helpful in clinical practice. Second, commercially available anterior segment measurement instruments could not image the posterior surface of the lens. Further study will be needed to investigate more ocular biometric parameters among more subjects.

In conclusion, children with myopia presented thinner and more posterior lenses, as well as smaller changes of anterior segment parameters and refraction after cycloplegia. The change of ACD was correlated with the change of refraction. This would help in better understanding the relationship between anterior segment and myopia.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Authors’ Contribution

Ying Yuan and Zhengwei Zhang equally contributed to this paper.


Grant information: this work was supported by the National Natural Science Foundation of China (Grant no. 81200713), the Foundation of Interdisciplinary Research of “Medical and Engineering or Science” of Shanghai Jiao Tong University (Grant no. YG2011MS62), the Natural Science Foundation of Shanghai, China (Grant no. 09ZR1425400), the Hospital Training Plan of Medical Talent in 2012 (12RC06), and Shanghai Key Laboratory of Fundus Disease.


  1. M. He, W. Huang, Y. Zheng, L. Huang, and L. B. Ellwein, “Refractive error and visual impairment in school children in rural southern China,” Ophthalmology, vol. 114, no. 2, pp. 374–482, 2007. View at Publisher · View at Google Scholar · View at Scopus
  2. M. He, J. Zeng, Y. Liu, J. Xu, G. P. Pokharel, and L. B. Ellwein, “Refractive error and visual impairment in urban children in southern China,” Investigative Ophthalmology and Visual Science, vol. 45, no. 3, pp. 793–799, 2004. View at Publisher · View at Google Scholar · View at Scopus
  3. Y.-H. Guo, H.-Y. Lin, L. L. K. Lin, and C.-Y. Cheng, “Self-reported myopia in Taiwan: 2005 Taiwan National Health Interview Survey,” Eye, vol. 26, no. 5, pp. 684–689, 2012. View at Publisher · View at Google Scholar · View at Scopus
  4. M. Dirani, Y.-H. Chan, G. Gazzard et al., “Prevalence of refractive error in Singaporean Chinese children: the strabismus, amblyopia, and refractive error in young Singaporean Children (STARS) study,” Investigative Ophthalmology & Visual Science, vol. 51, no. 3, pp. 1348–1355, 2010. View at Publisher · View at Google Scholar · View at Scopus
  5. S. Vitale, R. D. Sperduto, and F. L. Ferris III, “Increased prevalence of myopia in the United States between 1971–1972 and 1999–2004,” Archives of Ophthalmology, vol. 127, no. 12, pp. 1632–1639, 2009. View at Publisher · View at Google Scholar · View at Scopus
  6. C.-W. Pan, Y.-F. Zheng, A. R. Anuar et al., “Prevalence of refractive errors in a multiethnic Asian population: the Singapore epidemiology of eye disease study,” Investigative Ophthalmology and Visual Science, vol. 54, no. 4, pp. 2590–2598, 2013. View at Publisher · View at Google Scholar · View at Scopus
  7. Q. S. You, L. J. Wu, J. L. Duan et al., “Prevalence of myopia in school children in greater Beijing: the Beijing Childhood Eye study,” Acta Ophthalmologica, vol. 92, no. 5, pp. e398–e406, 2014. View at Google Scholar
  8. D. O. Mutti, K. Zadnik, R. E. Fusaro, N. E. Friedman, R. I. Sholtz, and A. J. Adams, “Optical and structural development of the crystalline lens in childhood,” Investigative Ophthalmology and Visual Science, vol. 39, no. 1, pp. 120–133, 1998. View at Google Scholar · View at Scopus
  9. K. Zadnik, D. O. Mutti, R. E. Fusaro, and A. J. Adams, “Longitudinal evidence of crystalline lens thinning in children,” Investigative Ophthalmology and Visual Science, vol. 36, no. 8, pp. 1581–1587, 1995. View at Google Scholar · View at Scopus
  10. H.-B. Wong, D. Machin, S.-B. Tan, T.-Y. Wong, and S.-M. Saw, “Ocular component growth curves among Singaporean children with different refractive error status,” Investigative Ophthalmology and Visual Science, vol. 51, no. 3, pp. 1341–1347, 2010. View at Publisher · View at Google Scholar · View at Scopus
  11. J. G. Sivak, “The role of the lens in refractive development of the eye: animal models of ametropia,” Experimental Eye Research, vol. 87, no. 1, pp. 3–8, 2008. View at Publisher · View at Google Scholar · View at Scopus
  12. B. Urban, M. Krȩtowska, M. Szumiński, and A. Bakunowicz-Łazarczyk, “Evaluation of anterior chamber depth measurements in emmetropic, hypermetropic and myopic eyes in children and adolescents using OCT Visante,” Klinika Oczna, vol. 114, no. 1, pp. 18–21, 2012. View at Google Scholar · View at Scopus
  13. L. Xu, W. F. Cao, Y. X. Wang, C. X. Chen, and J. B. Jonas, “Anterior chamber depth and chamber angle and their associations with ocular and general parameters: the Beijing Eye Study,” American Journal of Ophthalmology, vol. 145, no. 5, pp. 929–936.e1, 2008. View at Publisher · View at Google Scholar · View at Scopus
  14. J. Gwiazda, F. Thorn, J. Bauer, and R. Held, “Myopic children show insufficient accommodative response to blur,” Investigative Ophthalmology and Visual Science, vol. 34, no. 3, pp. 690–694, 1993. View at Google Scholar · View at Scopus
  15. D. O. Mutti, G. L. Mitchell, J. R. Hayes et al., “Accommodative lag before and after the onset of myopia,” Investigative Ophthalmology & Visual Science, vol. 47, no. 3, pp. 837–846, 2006. View at Google Scholar
  16. H. Shankar, D. Taranath, C. T. Santhirathelagan, and K. Pesudovs, “Repeatability of corneal first-surface wavefront aberrations measured with Pentacam corneal topography,” Journal of Cataract and Refractive Surgery, vol. 34, no. 5, pp. 727–734, 2008. View at Publisher · View at Google Scholar · View at Scopus
  17. G. L. van der Heijde and J. Weber, “Accommodation used to determine ultrasound velocity in the human lens,” Optometry and Vision Science, vol. 66, no. 12, pp. 830–833, 1989. View at Publisher · View at Google Scholar · View at Scopus
  18. E. L. Smith III, L.-F. Hung, B. Arumugam, and J. Huang, “Negative lens-induced myopia in infant monkeys: effects of high ambient lighting,” Investigative Ophthalmology and Visual Science, vol. 54, no. 4, pp. 2959–2969, 2013. View at Publisher · View at Google Scholar · View at Scopus
  19. S. R. Lambert, A. Fernandes, C. Drews-Botsch, and M. Tigges, “Pseudophakia retards axial elongation in neonatal monkey eyes,” Investigative Ophthalmology and Visual Science, vol. 37, no. 2, pp. 451–458, 1996. View at Google Scholar · View at Scopus
  20. R. Iribarren, I. G. Morgan, Y. H. Chan, X. Lin, and S.-M. Saw, “Changes in lens power in Singapore Chinese children during refractive development,” Investigative Ophthalmology and Visual Science, vol. 53, no. 9, pp. 5124–5130, 2012. View at Publisher · View at Google Scholar · View at Scopus
  21. L. Gao, X. Zhuo, A. K. H. Kwok, N. Yu, L. Ma, and J. Wang, “The change in ocular refractive components after cycloplegia in children,” Japanese Journal of Ophthalmology, vol. 46, no. 3, pp. 293–298, 2002. View at Publisher · View at Google Scholar · View at Scopus
  22. M. Palamar, Z. Alkan, S. Egrilmez, and A. Yagci, “Influences of tropicamide on anterior segment parameters with Pentacam in healthy individuals,” Journal of Ocular Pharmacology and Therapeutics, vol. 29, no. 3, pp. 349–352, 2013. View at Publisher · View at Google Scholar
  23. J. Bhatia, “Effect of tropicamide and homatropine eye drops on a-scan parameters of the phakic normal eyes,” Oman Medical Journal, vol. 26, no. 1, pp. 23–25, 2011. View at Publisher · View at Google Scholar · View at Scopus
  24. D. O. Mutti, K. Zadnik, S. Egashira, L. Kish, J. D. Twelker, and A. J. Adams, “The effect of cycloplegia on measurement of the ocular components,” Investigative Ophthalmology and Visual Science, vol. 35, no. 2, pp. 515–527, 1994. View at Google Scholar · View at Scopus
  25. S. Jeon, W. K. Lee, K. Lee, and N. J. Moon, “Diminished ciliary muscle movement on accommodation in myopia,” Experimental Eye Research, vol. 105, pp. 9–14, 2012. View at Publisher · View at Google Scholar · View at Scopus
  26. Y. Sun, S. Fan, H. Zheng, C. Dai, Q. Ren, and C. Zhou, “Noninvasive imaging and measurement of accommodation using dual-channel SD-OCT,” Current Eye Research, vol. 39, no. 6, pp. 611–619, 2014. View at Publisher · View at Google Scholar · View at Scopus
  27. I.-L. Tsai, C.-Y. Tsai, L.-L. Kuo, S.-W. Liou, S. Lin, and I.-J. Wang, “Transient changes of intraocular pressure and anterior segment configuration after diagnostic mydriasis with 1% tropicamide in children,” Clinical and Experimental Optometry, vol. 95, no. 2, pp. 166–172, 2012. View at Publisher · View at Google Scholar · View at Scopus
  28. N. Loewen and J.-C. Barry, “The use of cycloplegic agents. Results of a 1999 survey of German-speaking centers for pediatric ophthalmology and strabology,” Strabismus, vol. 8, no. 2, pp. 91–99, 2000. View at Publisher · View at Google Scholar · View at Scopus