Knocking Down Snrnp200 Initiates Demorphogenesis of Rod Photoreceptors in Zebrafish

Figure 2

(a) Quantification of normal, deformed, and dead zebrafish injected with control morpholinos (control-MO) () or Snrnp200-MO () from 2 to 4 days postfertilization (dpf). (b) The upper panel presents the regular and aberrant cDNA structures of the cbln1 gene, while the below panel indicates the aberrant splicing detected in the cbln1 gene in zebrafish injected with Snrnp200-MO. The first intron of the cbln1 gene was retained. (c) The relative expressions of splicing components, including prpf3 (), prpf8 (), prpf31 (), prpf6 (), eftud2 (), and sartl (), were upregulated in zebrafish injected with Snrnp200-MO when compared with those injected with control-MO. (d) The relative expressions of retina specific transcripts, including opn1lw1 (), gnat2 (), rs1 (), opnlswl (), snrpg (), rds4 (), gnb3 (), and rho (), were downregulated in zebrafish injected with Snrnp200-MO when compared with those injected with control-MO. Data in (a), (c), and (d) are presented as mean ± standard deviation (SD) for technical triplicates. ; .

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