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Journal of Ophthalmology
Volume 2015, Article ID 839137, 7 pages
http://dx.doi.org/10.1155/2015/839137
Research Article

Dye-Free Porcine Model of Experimental Branch Retinal Vein Occlusion: A Suitable Approach for Retinal Proteomics

1Department of Ophthalmology, Aalborg University Hospital, Hobrovej 18-22, 9000 Aalborg, Denmark
2Biomedical Research Laboratory, Aalborg University Hospital, Ladegårdsgade 3, 9000 Aalborg, Denmark
3Department of Heart and Lung Surgery, Center for Cardiovascular Research, Aalborg University Hospital, Hobrovej 18-22, 9000 Aalborg, Denmark
4Department of Health Science and Technology, Aalborg University, Fredrik Bajers Vej 3, Building B, 9220 Aalborg, Denmark
5Department of Biomedicine, Aarhus University, Ole Worms Allé 3, Building 1182, Room 024, 8000 Aarhus C, Denmark

Received 15 January 2015; Accepted 29 March 2015

Academic Editor: Theodore Leng

Copyright © 2015 Lasse Jørgensen Cehofski et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Branch retinal vein occlusion induces complex biological processes in the retina that are generated by a multitude of interacting proteins. These proteins and their posttranslational modifications can effectively be studied using modern proteomic techniques. However, no method for studying large-scale protein changes following branch retinal vein occlusion has been available until now. Obtainment of retinal tissue exposed to branch retinal vein occlusion is only available through experimental animal models. Traditional models of experimental branch retinal vein occlusion require the use of Rose Bengal dye combined with argon laser photocoagulation. The use of Rose Bengal dye is problematic in proteomic studies as the dye can induce multiple protein modifications when irradiated. This paper presents a novel technique for proteomic analysis of porcine retinal tissue with branch retinal vein occlusion combining a dye-free experimental model with label-free liquid chromatography mass spectrometry based proteomics.