Propolis, a Constituent of Honey, Inhibits the Development of Sugar Cataracts and High-Glucose-Induced Reactive Oxygen Species in Rat Lenses

Figure 1

Effect of propolis on the viability and ROS production of cultured RLECs. Effect of propolis on intracellular ROS production and survival of RLECs following high glucose stress. Honey (50 μg/mL; H50) or propolis (5 or 50 μg/mL; P5 or P50) was added to RLECs cultured with 5.5 or 55 mM D-glucose or with 55 mM D-mannitol as osmotic control; 120 hrs later, ROS levels were measured using H2-DCFH-DA assay (a) and cell viability was estimated using colorimetric MTS assay (b). Results shown are the mean ± SD of three experiments. Asterisks denote statistically significant differences. (a) , , and . (b) and .

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(b)