Preservation of Preloaded DMEK Lenticules in Dextran and Non-Dextran-Based Organ Culture Medium

Figure 3

Immunostaining results using ZO-1 marker for expression of tight junctional proteins in the preloaded DMEK lenticules visualized at 400x magnification. Preserved cells showed expression of these proteins confirming the integrity of the endothelial cells. However, ((a) and (c)) C1 and C3, respectively, showed some areas without endothelial cell borders and hence loss of tight junctions whereas (b) C2 expressed tight junction protein with less polymorphism compared to C1 and C3. Hexagonality of the cells was observed in C2.

(a)

(b)

(c)