Preservation of Preloaded DMEK Lenticules in Dextran and Non-Dextran-Based Organ Culture Medium

Figure 4

Cell apoptosis after preservation of preloaded DMEK lenticules. (a) Induced apoptosis on the control sample at 100x magnification. (b) C1 showed mild apoptosis whereas ((c) and (d)) C2 and C3 did not show any apoptosis after preservation and the nucleus was visible with methyl green counterstain at 100x magnification. Hence it was determined that the preservation of the DMEK lenticule in a cartridge for 4 days does not have any drastic damage on the endothelial cells.

(a)

(b)

(c)

(d)