SENP1-Mediated Desumoylation of DBC1 Inhibits Apoptosis Induced by High Glucose in Bovine Retinal Pericytes

Figure 3

SENP1 played an antagonistic role in BRPC apoptosis under high glucose conditions. (a) Analysis of cellular apoptosis levels in the BRPCs treatment groups for 48 h: NG+pcDNA, NG+HA-SENP1, HG+pcDNA, and HG+HA-SENP1. (b) Analysis of cellular apoptosis levels in the BRPCs treatment groups for 48 h: NG+Si-NC, NG+Si-SENP1, HG+Si-NC, and HG+Si-SENP1. (c) SENP1, Cleaved PARP, and BCL-2 protein expression profiles in the four groups for 48 h: NG+pcDNA, NG+HA-SENP1, HG+pcDNA, and HG+HA-SENP1. (d) SENP1, Cleaved PARP, and BCL-2 protein expression profiles in the four groups for 48 h: NG+Si-NC, NG+Si-SENP1, HG+Si-NC, and HG+Si-SENP1. Data are presented as their means ± SEM. Bars indicate SDs. versus NG+Si-NC or NG+pcDNA; versus HG+Si-NC or HG+pcDNA; versus NG+pcDNA; versus HG+pcDNA.

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