Comparative Analysis of KnockOut™ Serum with Fetal Bovine Serum for the In Vitro Long-Term Culture of Human Limbal Epithelial Cells

Figure 4

Expression of epithelial stem cell makers and differentiation markers in human limbal epithelial cells. The human limbal epithelial cells were strongly stained with ABCG2 and p63 (green) in SR and FAD media. The cells maintained in SR and FAD media showed weak staining for connexin 43 (Cx43) and cytokeratin 3 (K3). (a) SR medium and (b) FAD medium. Original magnification: (a) ×10; (b) ×10 (scale bars: 100 μm). The studies were carried out in triplicate, and representative figures are shown here.

(a)

(b)