Table of Contents Author Guidelines Submit a Manuscript
Table of Contents Alerts

To receive news and publication updates for Journal of Ophthalmology, enter your email address in the box below.

Journal of Ophthalmology
Volume 2016 (2016), Article ID 7304812, 10 pages
http://dx.doi.org/10.1155/2016/7304812
Research Article

Comparative Analysis of KnockOut™ Serum with Fetal Bovine Serum for the In Vitro Long-Term Culture of Human Limbal Epithelial Cells

Shaokun Zhang,1 Zaoxia Liu,2 Guanfang Su,2 and Hong Wu2

1Department of Orthopedics, The 1st Hospital of Jilin University, Changchun 130021, China
2Department of Ophthalmology, The 2nd Hospital of Jilin University, Changchun 130041, China

Received 25 March 2016; Revised 2 June 2016; Accepted 2 June 2016

Academic Editor: Vishal Jhanji

Copyright © 2016 Shaokun Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. A. Schermer, S. Galvin, and T.-T. Sun, “Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells,” Journal of Cell Biology, vol. 103, no. 1, pp. 49–62, 1986. View at Publisher · View at Google Scholar · View at Scopus
  2. G. Cotsarelis, S.-Z. Cheng, G. Dong, T.-T. Sun, and R. M. Lavker, “Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells,” Cell, vol. 57, no. 2, pp. 201–209, 1989. View at Publisher · View at Google Scholar · View at Scopus
  3. A. J. W. Huang and S. C. G. Tseng, “Corneal epithelial wound healing in the absence of limbal epithelium,” Investigative Ophthalmology and Visual Science, vol. 32, no. 1, pp. 96–105, 1991. View at Google Scholar · View at Scopus
  4. G. Pellegrini, O. Golisano, P. Paterna et al., “Location and clonal analysis of stem cells and their differentiated progeny in the human ocular surface,” Journal of Cell Biology, vol. 145, no. 4, pp. 769–782, 1999. View at Publisher · View at Google Scholar · View at Scopus
  5. R. A. Thoft and J. Friend, “The X, Y, Z hypothesis of corneal epithelial maintenance,” Investigative Ophthalmology and Visual Science, vol. 24, no. 10, pp. 1442–1443, 1983. View at Google Scholar · View at Scopus
  6. R. C. Buck, “Measurement of centripetal migration of normal corneal epithelial cells in the mouse,” Investigative Ophthalmology and Visual Science, vol. 26, no. 9, pp. 1296–1299, 1985. View at Google Scholar · View at Scopus
  7. T. R. M. Henderson, D. J. Coster, and K. A. Williams, “The long term outcome of limbal allografts: the search for surviving cells,” British Journal of Ophthalmology, vol. 85, no. 5, pp. 604–609, 2001. View at Publisher · View at Google Scholar · View at Scopus
  8. S. M. Daya, A. Watson, J. R. Sharpe et al., “Outcomes and DNA analysis of ex vivo expanded stem cell allograft for ocular surface reconstruction,” Ophthalmology, vol. 112, no. 3, pp. 470–477, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. J. G. Rheinwald and H. Green, “Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells,” Cell, vol. 6, no. 3, pp. 331–334, 1975. View at Publisher · View at Google Scholar · View at Scopus
  10. G. Pellegrini, C. E. Traverso, A. T. Franzi, M. Zingirian, R. Cancedda, and M. De Luca, “Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium,” The Lancet, vol. 349, no. 9057, pp. 990–993, 1997. View at Publisher · View at Google Scholar · View at Scopus
  11. Y. Barrandon and H. Green, “Three clonal types of keratinocyte with different capacities for multiplication,” Proceedings of the National Academy of Sciences of the United States of America, vol. 84, no. 8, pp. 2302–2306, 1987. View at Publisher · View at Google Scholar · View at Scopus
  12. A. Rochat, K. Kobayashi, and Y. Barrandon, “Location of stem cells of human hair follicles by clonal analysis,” Cell, vol. 76, no. 6, pp. 1063–1073, 1994. View at Publisher · View at Google Scholar · View at Scopus
  13. G. Pellegrini, R. Ranno, G. Stracuzzi et al., “The control of epidermal stem cells (holoclones) in the treatment of massive full-thickness burns with autologous keratinocytes cultured on fibrin,” Transplantation, vol. 68, no. 6, pp. 868–879, 1999. View at Publisher · View at Google Scholar · View at Scopus
  14. V. Ronfard, J.-M. Rives, Y. Neveux, H. Carsin, and Y. Barrandon, “Long-term regeneration of human epidermis on third degree burns transplanted with autologous cultured epithelium grown on a fibrin matrix,” Transplantation, vol. 70, no. 11, pp. 1588–1598, 2000. View at Publisher · View at Google Scholar · View at Scopus
  15. V. Barbaro, A. Testa, E. Di Iorio, F. Mavilio, G. Pellegrini, and M. De Luca, “C/EBPδ regulates cell cycle and self-renewal of human limbal stem cells,” Journal of Cell Biology, vol. 177, no. 6, pp. 1037–1049, 2007. View at Publisher · View at Google Scholar · View at Scopus
  16. E. Dellambra, O. Golisano, S. Bondanza et al., “Downregulation of 14-3-3σ prevents clonal evolution and leads to immortalization of primary human keratinocytes,” Journal of Cell Biology, vol. 149, no. 5, pp. 1117–1129, 2000. View at Publisher · View at Google Scholar · View at Scopus
  17. X. Zheng, H. Baker, W. S. Hancock, F. Fawaz, M. McCaman, and E. Pungor Jr., “Proteomic analysis for the assessment of different lots of fetal bovine serum as a raw material for cell culture. Part IV. Application of proteomics to the manufacture of biological drugs,” Biotechnology Progress, vol. 22, no. 5, pp. 1294–1300, 2006. View at Publisher · View at Google Scholar · View at Scopus
  18. L. Tuschong, S. L. Soenen, R. M. Blaese, F. Candotti, and L. M. Muul, “Immune response to fetal calf serum by two adenosine deaminase-deficient patients after T cell gene therapy,” Human Gene Therapy, vol. 13, no. 13, pp. 1605–1610, 2002. View at Publisher · View at Google Scholar · View at Scopus
  19. C. S. De Paiva, S. C. Pflugfelder, and D.-Q. Li, “Cell size correlates with phenotype and proliferative capacity in human corneal epithelial cells,” Stem Cells, vol. 24, no. 2, pp. 368–375, 2006. View at Publisher · View at Google Scholar · View at Scopus
  20. Z. Chen, C. S. De Paiva, L. Luo, F. L. Kretzer, S. C. Pflugfelder, and D.-Q. Li, “Characterization of putative stem cell phenotype in human limbal epithelia,” Stem Cells, vol. 22, no. 3, pp. 355–366, 2004. View at Publisher · View at Google Scholar · View at Scopus
  21. G. G. Gallico III, N. E. O'Connor, and C. C. Compton, “Permanent coverage of large burn wounds with autologous cultured human epithelium,” The New England Journal of Medicine, vol. 311, no. 7, pp. 448–451, 1984. View at Publisher · View at Google Scholar · View at Scopus
  22. G. Romagnoli, M. De Luca, F. Faranda et al., “Treatment of posterior hypospadias by the autologous graft of cultured urethral epithelium,” The New England Journal of Medicine, vol. 323, no. 8, pp. 527–530, 1990. View at Publisher · View at Google Scholar · View at Scopus
  23. R. J.-F. Tsai, L.-M. Li, and J.-K. Chen, “Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells,” The New England Journal of Medicine, vol. 343, no. 2, pp. 86–93, 2000. View at Publisher · View at Google Scholar · View at Scopus
  24. I. R. Schwab, “Cultured corneal epithelia for ocular surface disease,” Transactions of the American Ophthalmological Society, vol. 97, pp. 891–986, 1999. View at Google Scholar · View at Scopus
  25. N. Koizumi, T. Inatomi, T. Suzuki, C. Sotozono, and S. Kinoshita, “Cultivated corneal epithelial transplantation for ocular surface reconstruction in acute phase of Stevens-Johnson syndrome,” Archives of Ophthalmology, vol. 119, no. 2, pp. 298–300, 2001. View at Google Scholar · View at Scopus
  26. M. Grueterich, E. Espana, and S. C. G. Tseng, “Connexin 43 expression and proliferation of human limbal epithelium on intact and denuded amniotic membrane,” Investigative Ophthalmology and Visual Science, vol. 43, no. 1, pp. 63–71, 2002. View at Google Scholar · View at Scopus
  27. K. Nishida, M. Yamato, Y. Hayashida et al., “Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface,” Transplantation, vol. 77, no. 3, pp. 379–385, 2004. View at Publisher · View at Google Scholar · View at Scopus
  28. P. Rama, S. Matuska, G. Paganoni, A. Spinelli, M. De Luca, and G. Pellegrini, “Limbal stem-cell therapy and long-term corneal regeneration,” New England Journal of Medicine, vol. 363, no. 2, pp. 147–155, 2010. View at Publisher · View at Google Scholar · View at Scopus
  29. Y. Barrandon and H. Green, “Cell migration is essential for sustained growth of keratinocyte colonies: the roles of transforming growth factor-α and epidermal growth factor,” Cell, vol. 50, no. 7, pp. 1131–1137, 1987. View at Publisher · View at Google Scholar · View at Scopus
  30. G. Pellegrini, P. Rama, F. Mavilio, and M. De Luca, “Epithelial stem cells in corneal regeneration and epidermal gene therapy,” The Journal of Pathology, vol. 217, no. 2, pp. 217–228, 2009. View at Publisher · View at Google Scholar · View at Scopus
  31. D. G. Halme and D. A. Kessler, “FDA regulation of stem-cell-based therapies,” New England Journal of Medicine, vol. 355, no. 16, pp. 1730–1735, 2006. View at Publisher · View at Google Scholar · View at Scopus
  32. S. Yokoo, S. Yamagami, T. Usui, S. Amano, and M. Araie, “Human corneal epithelial equivalents for ocular surface reconstruction in a complete serum-free culture system without unknown factors,” Investigative Ophthalmology and Visual Science, vol. 49, no. 6, pp. 2438–2443, 2008. View at Publisher · View at Google Scholar · View at Scopus
  33. K. Lekhanont, L. Choubtum, R. S. Chuck, T. Sa-Ngiampornpanit, V. Chuckpaiwong, and A. Vongthongsri, “A serum- and feeder-free technique of culturing human corneal epithelial stem cells on amniotic membrane,” Molecular Vision, vol. 15, pp. 1294–1302, 2009. View at Google Scholar
  34. G. Pellegrini, E. Dellambra, O. Golisano et al., “p63 identifies keratinocyte stem cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 6, pp. 3156–3161, 2001. View at Publisher · View at Google Scholar · View at Scopus
  35. E. D. Iorio, V. Barbaro, A. Ruzza, D. Ponzin, G. Pellegrini, and M. De Luca, “Isoforms of ΔNp63 and the migration of ocular limbal cells in human corneal regeneration,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 27, pp. 9523–9528, 2005. View at Publisher · View at Google Scholar · View at Scopus
  36. U. Schlötzer-Schrehardt and F. E. Kruse, “Identification and characterization of limbal stem cells,” Experimental Eye Research, vol. 81, no. 3, pp. 247–264, 2005. View at Publisher · View at Google Scholar · View at Scopus
  37. C.-Y. Liu, G. Zhu, R. Converse et al., “Characterization and chromosomal localization of the cornea-specific murine keratin gene Krt1.12,” The Journal of Biological Chemistry, vol. 269, no. 40, pp. 24627–24636, 1994. View at Google Scholar · View at Scopus
  38. M. Matic, I. N. Petrov, S. Chen, C. Wang, S. D. Dimitrijevich, and J. M. Wolosin, “Stem cells of the corneal epithelium lack connexins and metabolite transfer capacity,” Differentiation, vol. 61, no. 4, pp. 251–260, 1997. View at Publisher · View at Google Scholar · View at Scopus
  39. Y. Dong, M. Roos, M. Gruijters et al., “Differential expression of two gap junction proteins in corneal epithelium,” European Journal of Cell Biology, vol. 64, no. 1, pp. 95–100, 1994. View at Google Scholar · View at Scopus
  40. J. M. Wolosin, X. Xiong, M. Schütte, Z. Stegman, and A. Tieng, “Stem cells and differentiation stages in the limbo-corneal epithelium,” Progress in Retinal and Eye Research, vol. 19, no. 2, pp. 223–255, 2000. View at Publisher · View at Google Scholar · View at Scopus