Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma Cells

Figure 3

Zeaxanthin inhibits migration of UM cells by using Boyden chamber assay. Cells (C918) were seeded into the upper chamber and the lower chamber was filled with DMEM with 10% serum (see Material and Methods). After being cultured with (b) or without (a) zeaxanthin at 10 μM for 8 h, the migration of UM cells was measured by counting the migrating cells on the lower surface of the membrane at 10 fields. Photos were taken by a light microscope at ×200. Numbers of migrating cells in cultures without zeaxanthin and with zeaxanthin were and cells (mean ± SD), respectively, and expressed at the bar graph as and (percentages of the control), respectively. Zeaxanthin significantly inhibited the migration of UM cells (, ) (c). , versus control (cells cultured without zeaxanthin).

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