Development of Poly Lactic/Glycolic Acid (PLGA) Microspheres for Controlled Release of Rho-Associated Kinase Inhibitor

Figure 2

Stability and safety assessment of Y-27632 released from PLGA microspheres. (a) Y-27632 incorporating poly lactic/glycolic acid (PLGA) microspheres was incubated in PBS, and PBS was recovered after 3 and 7 days. The amount of Y-27632 released in the PBS was added to a culture medium as final concentration of Y-27632 as 10 μM. Phase contrast images of corneal endothelial cells (CECs) cultured for 24 hours are shown. (b) CECs were seeded at a density of 5.0 × 10³ cells/cm² per well on a 96-well plate for 24 hours and subjected to serum starvation for an additional 24 hours supplemented with fresh Y-27632 (10 μM) or Y-27632 released from PLGA microspheres after 3 or 7 days (10 μM). Control cells were CECs cultured for an additional 24 hours without supplementation with Y-27632. The data represent average ± SE (). , . These experiments were performed in triplicate.

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