Reduced Expression of VAMP8 in Lacrimal Gland Affected by Chronic Graft-versus-Host Disease

Figure 2

Characteristics histopathological findings of lacrimal gland in a mouse model of cGVHD. Hematoxylin & eosin (H&E) staining for lacrimal gland tissue sections from GVHD-affected mouse model (a, c) and syngeneic control (b, d). Immunostaining for CD45⁺ cells (in green and red arrow) and cell nuclei (stained with 4,6-diamidino-2-phenylindole (DAPI) in blue) in the lacrimal gland collected from the GVHD-impaired mice (e) and syngeneic controls (f). Transmission electron microscopic (TEM) findings of the lacrimal gland in a mouse model of cGVHD (g, i) and the syngeneic controls (h, j). (g, h) Yellow arrows, secretory vesicles. Scale bar = 50 μm (a, b), 25 μm (c, d), 50 μm (e, f), 5 μm (g, h), and 0.5 μm (i, j). cGVHD mice, ; syngeneic control mice, . Representative figures from three independent experiments.