Reduced Expression of VAMP8 in Lacrimal Gland Affected by Chronic Graft-versus-Host Disease

Figure 3

VAMP8 and E-cadherin expression in cGVHD-impaired murine lacrimal gland. VAMP8 expression in the lacrimal gland epithelia affected by cGVHD (a) and in the syngeneic control subjects (b). E-cadherin expression in the lacrimal gland epithelia in the GVHD-affected mice ((c), red boxed area, reduction of E-cadherin expression) and syngeneic control (d). VAMP8 is shown in red, E-cadherin in green, and 4,6-diamidino-2-phenylindole (DAPI) in blue. Scale bar = 25 μm (a, b, c, and d). The vertical axis shows the average of fluorescence intensity of VAMP8 measured by ImageJ (e). The intensity of VAMP8 in the syngeneic control and GVHD-affected mice at the 3 and 8 weeks after bone marrow transplantation. . Bar = mean ± SD. , at 3 weeks, at 8 weeks. cGVHD mice, ; syngeneic control mice, . The experiments were repeated three times.

(a)

(b)

(c)

(d)

(e)