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Journal of Ophthalmology
Volume 2017 (2017), Article ID 4046597, 14 pages
Research Article

Changes in Retinal Function and Cellular Remodeling Following Experimental Retinal Detachment in a Rabbit Model

1Division of Ophthalmology, Rabin Medical Center, Beilinson Campus, Petah Tikva, Israel
2Laboratory of Eye Research, Felsenstein Medical Research Center (FMRC), Rabin Medical Center, Petah Tikva, Israel
3Koret School of Veterinary Medicine, The R. H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
4Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
5The Israeli National Hemophilia Center, Sheba Medical Center, Tel Hashomer, Israel

Correspondence should be addressed to Tilda Barliya

Received 30 November 2016; Revised 29 January 2017; Accepted 13 February 2017; Published 9 April 2017

Academic Editor: Toshihide Kurihara

Copyright © 2017 Tilda Barliya et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. To explore functional electroretinographic (ERG) changes and associated cellular remodeling following experimental retinal detachment in a rabbit model. Methods. Retinal detachment was created in ten rabbits by injecting 0.1 ml balanced salt solution under the retina. Fundus imaging was performed 0, 3, 7, 14, and 21 days postoperatively. ERGs were recorded pre- and 7 and 21 days postoperatively. Eyes were harvested on day 21 and evaluated immunohistochemically (IHC) for remodeling of second- and third-order neurons. Results. Retinal reattachment occurred within two weeks following surgery. No attenuation was observed in the photopic or scotopic a- and b-waves. A secondary wavefront on the descending slope of the scotopic b-wave was the only ERG result that was attenuated in detached retinas. IHC demonstrated anatomical changes in both ON and OFF bipolar cells. Bassoon staining was observed in the remodeled dendrites. Amacrine and horizontal cells did not alter, but Muller cells were clearly reactive with marked extension. Conclusion. Retinal detachment and reattachment were associated with functional and anatomical changes. Exploring the significance of the secondary scotopic wavefront and its association with the remodeling of 2nd- and 3rd-order neurons will shade more light on functional changes and recovery of the retina.