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Journal of Ophthalmology
Volume 2017 (2017), Article ID 4052094, 12 pages
https://doi.org/10.1155/2017/4052094
Research Article

Tissue Distribution of trans-Resveratrol and Its Metabolites after Oral Administration in Human Eyes

1Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jie-fang Road, Wuhan, Hubei Province, China
2Department of Ophthalmology, General Hospital of Wanbei Coal Group, Suzhou, Anhui 234000, China
3School of Chemistry and Engineering, Wuhan University of Technology, Wuhan 430070, China
4Eye Center, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310000, China
5Shiley Eye Institute and Department of Ophthalmology, University of California, San Diego, La Jolla, San Diego, CA, USA

Correspondence should be addressed to Xufang Sun

Received 12 November 2016; Accepted 30 January 2017; Published 20 March 2017

Academic Editor: Vicente Zanon-Moreno

Copyright © 2017 Shuaishuai Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. This study was performed to measure the concentration of trans-resveratrol and its three metabolites in human eyes. Methods. The patients who underwent pars plana vitrectomy for rhegmatogenous retinal detachment were included. The participants were orally given trans-resveratrol-based supplement (Longevinex®). A suitable amount of conjunctiva, aqueous humor, and vitreous humor were obtained during the operation. High-performance liquid chromatography (HPLC) with mass spectrometry (LC/MS/MS) was used to detect the concentration of trans-resveratrol and its three metabolites in the various samples. Results. The average concentration of resveratrol in the conjunctiva was 17.19 ± 15.32 nmol/g (mean ± SD). The concentration of resveratrol in the aqueous humor was close to the limit of detection, but its metabolites could be quantified. The concentrations of resveratrol metabolites in the aqueous humor can be detected. In the vitreous humor, the average concentration of resveratrol-3-O-sulfate was 62.95 ± 41.97 nmol/L. The sulfate conjugations of resveratrol were recovered in the conjunctiva, aqueous humor, and vitreous humor. Conclusions. Resveratrol and its three metabolites can be detected in the ocular tissues after oral administration. Although the concentration of parent resveratrol was low in the eyes, its metabolites could be detected and may have a role in the treatment of ocular diseases.