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Journal of Ophthalmology
Volume 2017, Article ID 6896390, 7 pages
https://doi.org/10.1155/2017/6896390
Research Article

Gene Expression Profiling of the Optic Nerve Head of Patients with Primary Open-Angle Glaucoma

1Department of Ophthalmology, The Fourth Hospital of Xi’an, Xi’an, Shaanxi 710004, China
2Department of Endocrinology, Shaanxi Provincial People’s Hospital, Xi’an, Shaanxi 710068, China

Correspondence should be addressed to Hui Li; moc.361@hlyfxs

Received 8 November 2016; Revised 1 February 2017; Accepted 14 February 2017; Published 6 April 2017

Academic Editor: Ji-jing Pang

Copyright © 2017 Xinrong Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. The pressure-induced axonal injury of the vulnerable ONH has led many researchers to view glaucoma from the perspective of the genetic basis of the angle of the ONH. However, genetic studies on POAG from this perspective are limited. Methods. Microarray dataset GSE45570 of the ONH of healthy individuals and POAG patients were downloaded from the Gene Expression Omnibus. After screening for the DEGs using the limma package, enrichment analysis was performed using DAVID. The DEG interaction network was constructed using cancer spider at BioProfiling.de. Thereafter, DEG-related TFs were predicted using TRANSFAC, and TF-DEG regulatory networks were visualized using Cytoscape. Results. Thirty-one DEGs were identified including 11 upregulated and 20 downregulated DEGs. Thereafter, gene ontology terms of nucleosome assembly, sensory perception and cognition, and pathway of signaling by GPCR were found to be enriched among the DEGs. Furthermore, DEG interaction and TF-DEG networks were constructed. NEUROD1 was present in both the DEG network and the TF-DEG network as the node with the highest degree and was predicted as a marker gene in the ONH of patients with POAG. Conclusion. NEUROD1 may contribute greatly to the ONH of patients with POAG and was found to be involved in eye development and diseases.