Research Article
Inhibition of Rho Kinase Induces Antioxidative Molecules and Suppresses Reactive Oxidative Species in Trabecular Meshwork Cells
Figure 1
(a) Quantitative PCR analysis of catalase mRNA. The TM cells were treated with 25 μM Y-27632 for 30 min. The relative expression level of catalase of samples treated with Y-27632 was compared to that of the control sample using the comparative CT method (ΔΔCT method). The 18S ribosomal RNA was used as an endogenous control. Data are shown as mean ± SE from six independent experiments. compared with control by Wilcoxon rank sum test. (b) The effects of Y-27632 on the intracellular production of reactive oxygen species (ROS). The TM cells were treated with or without 25 μM Y-27632 for 30 min, followed by 100 μM menadione stimulated for 1 h. ROS were detected by CellROX reagent, and the fluorescence of the TM cells were measured by cell sorter SH800. Data are shown as mean ± SE from five independent experiments. and compared with control by the Wilcoxon rank sum test (a) and Tukey Kramer HSD test (b).
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