Inhibition of Rho Kinase Induces Antioxidative Molecules and Suppresses Reactive Oxidative Species in Trabecular Meshwork Cells

Figure 2

(a) The effect of Y-27632 on oxidative stress-induced cell death. The TM cells were treated with or without 25 μM Y-27632 for 30 min, followed by menadione stimulation of the cells for 24 h. Cell viabilities were shown as relative value compared with the control. Data are shown as the mean ± SE from six independent experiments. compared with control by Wilcoxon rank sum test. (b) The effect of Y-27632 on extracellular antioxidative activity. The xanthine-oxidase-induced superoxide production was assessed using a superoxide-sensitive luminescent reagent. Data are shown as the mean ± SE from six independent experiments. compared with the control by Dunnett’s test. Nac: n-acetyl cysteine.

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