Flt3 Regulation in the Mononuclear Phagocyte System Promotes Ocular Neovascularization

Figure 8

CD11c and CD206 levels assessed by quantitative PCR and immunofluorescence in retinas of CNV mouse model at D14. Quantification of CD11c and CD206 levels in mouse retinas are shown, respectively, in (A) and (B). Data are mean ± SE from three independent experiments (, ). Flt3−/− and wild-type C57BL/6 mice were submitted to laser-induced CNV. Double labeling of NV (green) with CD11c (red) or CD206 (red) was performed on choroidal flat mounts of mice after laser treatment. Immunofluorescence signals of CD11c are shown in (D) and (F); CD206 are shown in (I) and (K). Mean CD11c(+) and CD206(+) cell areas per CNV lesion are presented. Flt3−/− mice exhibited significantly decreased areas of CD11c staining and increased areas of CD206 staining in comparison with controls (); similar results were obtained at the mRNA level by real-time RT-PCR.