Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering

Figure 7

Xenograft of decellularized lenticules into the rabbit corneal stromal layer. DCL was inserted into the stromal layer and fixed with a suture. The slit lamp image shows that the transplanted decellularized lenticule is cleared at 2 weeks after operation. The dotted area indicates the inserted site of the lenticule (a). OCT image showing a transplanted DCL in the stromal layers at 2 (b) and 4 (d) weeks after operation. The DCL was located at the stromal layer. Hematoxylin and eosin staining was performed to detect infiltration of immune cells. There was no infiltration of immune cells in or around the DCL at up to 4 weeks (c). Original magnification is ×200, and scale bar indicates 50 μm (c). Implanted DCL.

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