Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering

Table 1

Decellularizing process. Corneal lenticules were decellularized by various conditioned solutions. A lenticule incubated with each decellularizing solution, followed by washing with PBS. The lenticules were incubated with DNase I and RNase A to remove nucleic acid. After washing with each tonic buffer, the lenticules were stored in Optisol at 4°C until use.

Number	Process	Time	Temp.
1	Washing with PBS	1 h	37°C
2	Incubation with each decellularizing solution	48 h	37°C
3	Washing with each tonic buffer	1 h	37°C
4	Incubation with DNase I and RNase A	24 h	37°C
5	Washing with each tonic buffer	1 h	37°C
6	Store in Optisol		4°C