Ability of Swept-Source Optical Coherence Tomography to Detect Retinal and Choroidal Changes in Patients with Multiple Sclerosis

Garcia-Martin, Elena; Jarauta, Laura; Vilades, Elisa; Ara, Jose Ramon; Martin, Jesus; Polo, Vicente; Larrosa, Jose Manuel; Pablo, Luis Emilio; Satue, Maria

doi:https://doi.org/10.1155/2018/7361212

Journal of Ophthalmology

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Abstract Introduction Methods Results Discussion Conclusions Data Availability Conflicts of Interest Acknowledgments Supplementary Materials References Copyright Related Articles

Research Article | Open Access

Volume 2018 | Article ID 7361212 | https://doi.org/10.1155/2018/7361212

Ability of Swept-Source Optical Coherence Tomography to Detect Retinal and Choroidal Changes in Patients with Multiple Sclerosis

Elena Garcia-Martin,^1,2Laura Jarauta,^2,3Elisa Vilades,^1,2Jose Ramon Ara,^2,4Jesus Martin,^2,4Vicente Polo,^1,2Jose Manuel Larrosa,^1,2Luis Emilio Pablo,^1,2and Maria Satue^1,2

Academic Editor: Inés Contreras

Received30 May 2018

Accepted09 Oct 2018

Published13 Nov 2018

Abstract

Purpose. To evaluate the ability of new swept-source (SS) optical coherence tomography (OCT) technology to detect changes in retinal and choroidal thickness in patients with multiple sclerosis (MS). Methods. A total of 101 healthy and 97 MS eyes underwent retinal and choroidal assessment using SS Triton OCT (Topcon). Macular thickness and peripapillary data (retinal, ganglion cell layer (GCL+, GCL++) and retinal nerve fiber layer (RNFL) thickness) were analyzed, including choroidal thickness evaluation. Results. Significant macular thinning was observed in all ETDRS areas () in MS patients. Peripapillary retinal, RNFL, and GCL ++ thickness showed a significant reduction in patients in all sectors () except in the nasal quadrant/sector (). GCL+ measurements were found to be reduced in the nasal (), inferonasal (), and temporal () sectors and total thickness (). Choroidal thickness was reduced in the outer macular ring in MS patients compared with controls (). Conclusion. New swept-source technology for OCT devices detects retinal thinning in MS patients, providing increased depth analysis of the choroid in these patients. MS patients present reduced retinal and choroidal thickness in the macular area and reduced peripapillary retinal, RNFL, and GCL thickness.

1. Introduction

Optic nerve atrophy and thinning of the peripapillary retinal nerve fiber layer (RNFL) are two typical findings of patients with multiple sclerosis (MS). Axonal damage already occurs in the early stages of the disease, and its relationship with demyelination is still unclear [1, 2]. Axonal damage is considered to be the main cause of disability in MS [3–5] and can be detected and quantified at the level of the retinal nerve fiber layer (RNFL) using ocular imaging technologies, such as optical coherence tomography (OCT) [5–10]. So far, studies using spectral-domain OCT have revealed that the retina in nonoptic neuritis (non-ON) eyes shows thinner peripapillary RNFL (pRNFL) than healthy controls [4, 11–13].

Digital imaging technologies in ophthalmology have greatly improved in the recent years. The most recent milestone in the development of retina and choroid structural visualization strategies is swept-source (SS) OCT, which overcomes the scattering of light on the choroid thanks to longer wavelengths than those used in SD systems (1,050 nm vs 840 nm) [14]. The scan speed in SS-OCT devices is of 100,000 A scans/sec, providing more accurate three-dimensional images of the retina and choroid [14, 15].

In the present study, we evaluated retinal and choroidal thickness in patients with MS without previous ON episodes (that is, without acute loss of RNFL thickness), using new SS-deep range imaging (DRI) OCT technology and compared obtained measurements with a group of healthy subjects. To the best of our knowledge, this is the first study evaluating MS eyes using new SS-DRI OCT technology.

2. Methods

All procedures in this study adhered to the tenets of the Declaration of Helsinki; the experimental protocol was approved by the Ethics Committee of the Miguel Servet Hospital (CEICA), and all participants provided written informed consent to participate in the study.

Patients with definite relapsing-remitting (RR) MS were included in an observational cross-sectional study. The sample size was calculated in order to detect significant differences in the RNFL, assuming an α error of 5% and a β error of 10%. Based on these calculations, the number of subjects needed was at least 80. A total of 97 eyes of 97 patients and 100 eyes of 100 healthy individuals were evaluated.

The diagnosis of MS was based on the McDonald criteria [16] and confirmed by a neurologist. Related medical records (neurological and ophthalmological) were carefully evaluated, and information about Expanded Disability Status Scale (EDSS) scores, disease duration and subtype, modifying disease treatments, and prior episodes ON (based on the standardized definition of ON using clinical criteria [17]) were recorded. Only patients with relapsing-remitting MS were included in our study. Patients with visual acuity <0.1 (6/60, using the Snellen chart) and intraocular pressure >20 mmHg and/or active MS outbreaks (of any neurologic deficit) in the 6 months preceding enrollment in the study were excluded from the study. The reason to exclude active neurological flare ups was that acute axonal losses did not mask neuronal damage secondary to MS progression (i.e., chronic neurodegeneration). Patients with previous ON history were excluded from the study because we aimed at evaluating the ability of Triton OCT to detect subclinical axonal damage in the RNFL of these patients compared to controls. Axial length was assessed in all individuals. Eyes longer than 25.2 mm and refractive errors ≥5 diopters (D) of spherical equivalent or ≥3 D of astigmatism were excluded from the study.

Structural measurements of the retina were obtained using the DRI Triton SS-OCT device (Topcon, Tokyo, Japan) which uses a tunable laser as a light source to provide a 1050 nm centered wavelength. This device reaches a scanning speed of 100,000 A-scans per second, yielding 8 and 20 μm axial and transverse resolution in tissue, respectively. The 3D wide protocol was used for all subjects. This protocol includes a wide scanning range that focuses both in the macular (ETDRS scan) and the peripapillary area (TSNIT). The diameter of the peripapillary area measured with this protocol is 3.40 mm. With the ETDRS scan nine macular areas [18] (which include a central 1 mm circle representing the fovea, and inner and outer rings measuring 3 mm and 6 mm in diameter, respectively), central and average thickness plus macular volume are analyzed; the TSNIT scan provides automated separate measurements of different retinal layers: retinal nerve fiber layer (RNFL) (between the inner limiting membrane (ILM)to the GCL boundaries), ganglion cell layer (GCL) + (between RNFL to the inner nuclear layer boundaries), GCL++ (between ILM to the inner nuclear layer boundaries), and retinal thickness (from the ILM to the retinal pigment epithelium boundaries). The TSNIT provides measurements of the 4 peripapillary quadrants (superior, nasal, inferior, and temporal), 6 sectors (superonasal, superotemporal, nasal, temporal, inferonasal, and inferotemporal), and 12 clock sectors. Additionally, both EDTRS and TSNIT protocols provide automated choroidal thickness measurements (from the Bruch membrane to the choroidal-scleral interface, Figure 1).

In this study, we followed the methods of Satue et al. 2017 [19]. Macular (ETDRS, GCL+, and GCL++) and peripapillary (RNFL, GCL+, and GCL++) thickness were evaluated. Choroidal measurements were obtained for both the macular (ETDRS) and peripapillary area (TSNIT).

All variables were registered in a database created with Excel 2010 (Microsoft Corporation). Modifier variables were age, sex, intraocular pressure, and axial length. Statistical analysis was performed using commercial predictive analytics software (SPSS, version 20.0; SPSS, Inc., Chicago, IL). The normality of the sample distribution was confirmed using the Kolmogorov–Smirnov test (). Bonferroni correction for multiple comparisons was applied. Comparisons between patients and controls were calculated using Student’s t test. value ≤0.05 was considered of statistical significance. Results of calculations are reported as “mean” and “standard deviation.” Only one eye per patient was randomly selected for the study.

3. Results

Ninety-seven eyes of 97 RR MS patients with a mean age of 48.46 years (SD = 11.31) and 101 eyes of 101 healthy individuals with a mean age of 47.58 years (SD = 9.37) were included in the study. The male/female ratio was 1/11 in the MS group (8/89) and 1/10 in the control group (9/91). Mean axial length was 23.52 ± 0.6 mm in the control group, and 23.60 ± 0.2 mm in the MS group. Mean age, sex, intraocular pressure, and axial length did not differ significantly between the groups (, respectively). Mean disease duration in the group of patients was 7.56 years (SD = 2.66). The median EDSS score was 1.50 (interquartile range (IQR) = 2.30), and all patients suffered from relapsing-remitting MS subtype. No previous acute optic neuritis attack was reported in any of our patients.

Measurements obtained with SS DRI Triton OCT showed reduced retinal thickness in all macular ETDRS sectors in MS patients compared with controls (). Macular volume was also significantly reduced in patients (7.92 mm³ in controls vs 7.54 mm³ in patients, ). Results can be observed in Table 1.

Significant peripapillary retinal and RNFL thinning was observed in all measured areas (total thickness, quadrants, and sectors, ) except in the nasal quadrant/sector (Table 2).

The ganglion cell layer as measured from the RNFL to the boundaries of the inner nuclear layer (GCL+) was found to be reduced in the nasal (40.96 µm in controls vs 38.96 µm in patients, ) and temporal (52.31 µm vs 49.20 µm, ) quadrants, in the nasal (40.96 µm vs 38.96 µm, ), inferonasal (37.51 µm vs 35.57 µm, ), and temporal (52.31 µm vs 49.20 µm, ) sectors, and in total thickness (43.20 µm vs 41.21 µm, ) (Table 2).

Automated measurements of the macular and peripapillary choroidal thickness in MS patients did not show any significant differences compared with healthy controls. However, a clear tendency toward reduced choroidal thickness was observed in the peripapillary area (Table 3). This tendency was not observed in the ETDRS macular choroidal measurements (Supplementary Table 1). A secondary analysis of the ETDRS choroidal thickness was performed due to high standard deviation values. The four areas of the 3 mm inner ETDRS ring (superior, nasal, inferior, and temporal) were assembled and calculated as only one area (inner ring), and the four areas of the 6 mm outer ETDRS ring (superior, nasal, inferior and temporal) were calculated as other unique area (outer ring). The outer ring of the choroidal plexus was found to be significantly reduced in MS patients compared with controls (261.41 µm in controls vs 254.46 µm in patients, ).

4. Discussion

In the present study, we evaluated retinal and choroidal changes in MS patients using new SS DRI Triton OCT device. To the best of our knowledge, this is the first study assessing the ability of Swept-source OCT technology to detect retinal changes in MS. Triton SS-OCT analyzed retinal thickness in both the macular and peripapillary area and also two other retinal layers: RNFL and GCL + (both in the peripapillary area). Triton SS-OCT detected significant retinal thinning in all the ETDRS areas in our patients, as well as retinal and RNFL thinning in all sectors of the peripapillary measurements (except in the nasal quadrant/sector). Surprisingly, ganglion cell layer measurements (GCL+) only showed significant reduction in the nasal and temporal quadrants/sectors.

Previous studies using SD-OCT segmentation analysis software demonstrated a reduction of the macular inner retinal layers, including the GCL + IPL, in patients with MS, suggesting ganglion cell loss [20–23]. Recent research demonstrated that GCL measurements as obtained with SD-OCT devices may be a better marker for axonal degeneration in MS compared with RNFL thickness [23]. In a recent study comparing both macular GCL + IPL and peripapillary RNFL thickness, average GCL + IPL was altered more frequently than average peripapillary RNFL, and GCL + IPL thickness demonstrated to have better sensitivity than temporal peripapillary RNFL thickness for detecting retinal thickness changes in patients with MS [12, 24]. Our study did not include macular GCL + thickness. However, in our patients, peripapillary RNFL thickness was altered more frequently than peripapillary GCL measurements (GCL+), and the latter were more reduced in temporal and nasal areas. The fact that the nasal quadrant is affected only in the GCL + in our patients (and not in the RNFL, GCL++) may suggest that, indeed, GCL measurements are somewhat a more subtle marker for neurodegeneration than RNFL thickness. However, more studies with SS-OCT technology including macular GCL + measurements are needed to corroborate these findings.

The choroid (present both in the ocular globe and the central nervous system) is composed of epithelial cells resting on a basal lamina. These epithelial cells produce the cerebrospinal fluid, which has many functions, such as providing mechanical support, a route for some nutrients and removing by-products of metabolism and synaptic activity [25]. The tight junctions located in the choroidal epithelial cells of the central nervous system form the blood-cerebrospinal fluid barrier. Previous atrophy of the choroidal epithelial cells and thickening of the basement membrane were observed in the central nervous system’s choroidal plexus of patients with other neurodegenerative diseases, such as Alzheimer’s disease [25]. In MS, an inflammatory disease, pathogenic autoreactive T lymphocytes may migrate through the blood-cerebrospinal fluid barrier of the choroid into the central nervous system [25]. Few previous studies focused on ocular choroidal thickness measurements in MS using OCT imaging, and none of them included SS technology. Esen et al. demonstrated, using enhanced depth imaging (EDI) SD OCT, that the subfoveal choroidal plexus in these patients was significantly reduced compared with healthy individuals, and this reduction was associated with disease duration [26]. Our patients showed significant reduced choroidal thickness in the assembled outer ring of the macular area, but not when the 9 separate EDTRS areas were compared. Additionally, the peripapillary choroidal plexus seemed to be diminished in our patients compared with controls (although this finding was not significant). Choroidal thickness is influenced by the axial length of the eye. Both refractive errors and axial length were evaluated in our study and did not differ significantly between patients and controls. Recent research comparing SD-OCT and SS-OCT choroidal measurements in healthy and high myopic subjects demonstrated that SS-OCT measurements provide better quality of choroidal images, which allows higher rates of measurement of this layer [15, 27]. However, choroidal measurements in our study were automatically obtained, which could add segmentation artefacts, but also decrease bias induced by manual measurement. In our opinion, more studies comparing SD-OCT and SS-OCT choroidal measurements in MS patients are needed to corroborate our findings.

A possible limitation is that subclinical glaucomatous eyes might have been included in the study, despite all participants (MS and controls) being evaluated for IOP levels (but not for glaucomatous changes in perimetry). It is possible that both the patient and the control group of subjects contained subclinical glaucomatous eyes. However, since this would have randomly affected both comparative groups, we do not consider this to alter significantly the results of our study. Another possible limitation is that the quality of some of the scans would have caused the choroidal thickness in the MS to be diminished when automated segmentation was applied. Nevertheless, we checked the quality of the scans immediately after acquisition, all quality scores were >55 in both groups, and no statistical difference between the MS and the control group was observed ()

5. Conclusions

New swept-source technology for OCT devices detects macular thinning and peripapillary retinal, RNFL, and GCL reduction in MS patients, providing increased and more detailed evaluation of the choroid in these patients. Based on our findings, SS-OCT devices could be used as an alternative tool to SD-technology in the routine evaluation of patients with MS. Moreover, since SS-OCT provides increased depth analysis of the choroid in these patients, analyzing the ocular choroidal plexus in MS subjects could add valuable information on the possible affectation of the ocular layers and reflect degeneration in other central nervous system structures. However, similar studies comparing new swept-source and SD OCT technology for the evaluation of MS would still be needed to corroborate our findings.

Data Availability

Raw data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of Interest

The authors have no proprietary or commercial interest in any materials discussed in this article.

Acknowledgments

This research was conducted at Miguel Servet University Hospital (Zaragoza, Spain) and received no specific funding by any agency in the public, commercial, or not-for-profit sectors. MS was supported by a fellowship from the Instituto de Salud Carlos III, Spain (Rio Hortega program: CM14/00026). Publishing costs were supported by Instituto de Salud Carlos III, Spain (grant number PI17/01726). This research was supported by FEDER (European fund for regional development) “Otra manera de hacer Europa.”

Supplementary Materials

Supplementary Table 1: Macular choroidal thickness as measured with Triton optical coherence tomography, in patients with multiple sclerosis and controls. (Supplementary Materials)

References

J. Palace, “Inflammation versus neurodegeneration: consequences for treatment,” Journal of the Neurological Sciences, vol. 259, no. 1-2, pp. 46–49, 2007.
View at: Publisher Site | Google Scholar
S. L. Hauser and J. R. Oksenberg, “The neurobiology of multiple sclerosis: genes, inflammation, and neurodegeneration,” Neuron, vol. 52, no. 1, pp. 61–76, 2006.
View at: Publisher Site | Google Scholar
J. B. Fisher, D. A. Jacobs, C. E. Markowitz et al., “Relation of visual function to retinal nerve fiber layer thickness in multiple sclerosis,” Ophthalmology, vol. 113, no. 2, pp. 324–332, 2006.
View at: Publisher Site | Google Scholar
E. Gordon-Lipkin, B. Chodkowski, D. S. Reich et al., “Retinal nerve fiber layer is associated with brain atrophy in multiple sclerosis,” Neurology, vol. 69, no. 16, pp. 1603–1609, 2007.
View at: Publisher Site | Google Scholar
E. Garcia-Martin, V. Pueyo, I. Pinilla, J. R. Ara, J. Martin, and J. F. ernandez, “Fourier-domain OCT in multiple sclerosis patients: reproducibility and ability to detect retinal nerve fiber layer atrophy,” Investigative Opthalmology and Visual Science, vol. 52, no. 7, pp. 4124–4131, 2011.
View at: Publisher Site | Google Scholar
A. A. Khanifar, G. J. Parlitsis, J. R. Ehrlich et al., “Retinal nerve fiber layer evaluation in multiple sclerosis with spectral domain optical coherence tomography,” Clinical Ophthalmology, vol. 4, pp. 1007–1013, 2010.
View at: Google Scholar
R. C. Sergott, “Optical coherence tomography: measuring in-vivo axonal survival and neuroprotection in multiple sclerosis and optic neuritis,” Current Opinion in Ophthalmology, vol. 16, no. 6, pp. 346–350, 2005.
View at: Publisher Site | Google Scholar
M. S. Zaveri, A. Conger, A. Salter et al., “Retinal imaging by laser polarimetry and optical coherence tomography evidence of axonal degeneration in multiple sclerosis,” Archives of Neurology, vol. 65, no. 7, pp. 924–928, 2008.
View at: Publisher Site | Google Scholar
F. C. Gundogan, S. Demirkaya, and G. Sobaci, “Is optical coherence tomography really a new biomarker candidate in multiple sclerosis? A structural and functional evaluation,” Investigative Opthalmology and Visual Science, vol. 48, no. 12, pp. 5773–5781, 2007.
View at: Publisher Site | Google Scholar
E. Garcia-Martin, V. Pueyo, J. Martin et al., “Progressive changes in the retinal nerve fiber layer in patients with multiple sclerosis,” European Journal of Ophthalmology, vol. 20, no. 1, pp. 167–173, 2010.
View at: Publisher Site | Google Scholar
J. Dorr, K. D. Wernecke, M. Bock et al., “Association of retinal and macular damage with brain atrophy in multiple sclerosis,” PLoS One, vol. 6, no. 4, Article ID e18132, 2011.
View at: Publisher Site | Google Scholar
J. J. González-López, G. Rebolleda, M. Leal et al., “Comparative diagnostic accuracy of ganglion cell-inner plexiform and retinal nerve fiber layer thickness measures by Cirrus and Spectralis optical coherence tomography in relapsing-remitting multiple sclerosis,” BioMed Research International, vol. 2014, Article ID 128517, 10 pages, 2014.
View at: Publisher Site | Google Scholar
A. J. Green, S. McQuaid, S. L. Hauser, I. V. Allen, and R. Layness, “Ocular pathology in multiple sclerosis: retinal atrophy and inflammation irrespective of disease duration,” Brain, vol. 133, no. 6, pp. 1591–1601, 2010.
View at: Publisher Site | Google Scholar
M. Hirata, A. Tsujikawa, A. Matsumoto et al., “Macular choroidal thickness and volume in normal subjects measured by swept-source optical coherence tomography,” Investigative Opthalmology and Visual Science, vol. 52, no. 8, pp. 4971–4978, 2011.
View at: Publisher Site | Google Scholar
S. Copete, I. Flores-Moreno, J. A. Montero, J. S. Duker, and J. M. Ruiz-Moreno, “Direct comparison of spectral-domain and swept-source OCT in the measurement of choroidal thickness in normal eyes,” British Journal of Ophthalmology, vol. 98, no. 3, pp. 334–338, 2014.
View at: Publisher Site | Google Scholar
C. H. Polman, S. C. Reingold, G. Edan et al., “Diagnostic criteria for multiple sclerosis: 2005 revisions to the “McDonald Criteria”,” Annals of Neurology, vol. 58, no. 6, pp. 840–846, 2005.
View at: Publisher Site | Google Scholar
A. Petzold, M. P. Wattjes, F. Costello et al., “The investigation of acute optic neuritis: a review and proposed protocol,” Nature Reviews Neurology, vol. 10, no. 8, pp. 447–458, 2014.
View at: Publisher Site | Google Scholar
Early Treatment Diabetic Retinopathy Study Research Group, “Photocoagulation for diabetic macular edema. Early treatment diabetic retinopathy study report number 1,” Archives of Ophthalmology, vol. 103, no. 12, pp. 1796–1806, 1985.
View at: Google Scholar
M. Satue, J. Obis, R. Alarcia et al., “Retinal and choroidal changes in patients with Parkinson's disease detected by swept source optical coherence tomography,” Current Eye Research, vol. 43, no. 1, pp. 109–115, 2018.
View at: Publisher Site | Google Scholar
S. Saidha, S. B. Syc, M. K. Durbin et al., “Visual dysfunction in multiple sclerosis correlates better with optical coherence tomography derived estimates of macular ganglion cell thickness than peripapillary retinal nerve fiber layer thickness,” Multiple Sclerosis Journal, vol. 17, no. 12, pp. 1449–1463, 2011.
View at: Publisher Site | Google Scholar
S. D. Walter, H. Ishikawa, K. M. Galetta et al., “Ganglion cell loss in relation to visual disability in multiple sclerosis,” Ophthalmology, vol. 119, no. 6, pp. 1250–1257, 2012.
View at: Publisher Site | Google Scholar
E. C. Davies, K. M. Galetta, D. J. Sackel et al., “Retinal ganglion cell layer volumetric assessment by spectral-domain optical coherence tomography in multiple sclerosis: application of a high precision manual estimation technique,” Journal of Neuro-Ophthalmology, vol. 31, no. 3, pp. 260–264, 2011.
View at: Publisher Site | Google Scholar
E. Garcia-Martin, V. Polo, J. M. Larrosa et al., “Retinal layer segmentation in patients with multiple sclerosis using spectral domain optical coherence tomography,” Ophthalmology, vol. 121, no. 2, pp. 573–579, 2014.
View at: Publisher Site | Google Scholar
D. Narayanan, H. Cheng, K. N. Bonem, R. Saenz, R. A. Tang, and L. J. Frishman, “Tracking changes over time in retinal nerve fiber layer and ganglion cell-inner plexiform layer thickness in multiple sclerosis,” Multiple Sclerosis Journal, vol. 20, pp. 1331–1341, 2014.
View at: Publisher Site | Google Scholar
C. Kaur, G. Rathnasamy, and E. A. Ling, “The choroid plexus in healthy and diseased brain,” Journal of Neuropathology and Experimental Neurology, vol. 75, no. 3, pp. 198–213, 2016.
View at: Publisher Site | Google Scholar
E. Esen, S. Sizmaz, T. Demir, M. Demirkiran, I. Unal, and N. Demircan, “Evaluation of choroidal vascular changes in patients with multiple sclerosis using enhanced depth imaging optical coherence tomography,” Ophthalmologica, vol. 235, no. 2, pp. 65–71, 2016.
View at: Publisher Site | Google Scholar
L. S. Lim, G. Cheung, and S. Y. Lee, “Comparison of spectral domain and swept-source optical coherence tomography in pathological myopia,” Eye, vol. 28, no. 4, pp. 488–491, 2014.
View at: Publisher Site | Google Scholar

Copyright

Copyright © 2018 Elena Garcia-Martin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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