Research Article
DNA Methylation Status of the Interspersed Repetitive Sequences for LINE-1, Alu, HERV-E, and HERV-K in Trabeculectomy Specimens from Glaucoma Eyes
Figure 1
Combined-bisulfite restriction analysis (COBRA) for LINE-1 (a, b) and Alu (c, d) methylation patterns. The diagrams (a, c) demonstrate the following four patterns of methylated CpGs (from top to bottom): hypermethylation (mCmC), hypomethylation (uCuC), and two forms of partial methylation (mCuC and uCmC). Polyacrylamide gel electrophoresis (b, d) shows the locations for the bands for each pattern of methylated CpGs. Quantitative DNA ladder was used to assess the size of the bands. The representative gels of cell line and normal control samples are shown. Water is used as a negative control.
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