Overexpression of MiR-183/96/182 Triggers Retina-Like Fate in Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMSCs) in Culture

Figure 5

Evaluation of the expression of recoverin, RHO, and SLC1A1 in hBMSCs transfected with miR-183/96/182 mimics and scramble. (a) The qRT-PCR showed significant upregulation of recoverin and RHO mRNAs in the transfected cells after 24 hours. (b) The qRT-PCR revealed significant upregulation of recoverin mRNAs in the transfected cells after 48 hours. However, SLC1A1 did not lead to any significant differences in expression between transfected cells with miR-183/96/182 compared to transfected cells with scramble after 24 and 48 hours. The data were then normalized to GAPDH. All the data represented the mean ± standard deviation (SD) of the three independent experiments with n = 3.

(a)

(b)