LncRNA FEZF1-AS1 Promotes TGF-β2-Mediated Proliferation and Migration in Human Lens Epithelial Cells SRA01/04

Figure 1

FEZF1-AS1 is upregulated, accompanied by the increased proliferation and migration of TGF-β2-treated SRA01/04 cells. The SRA01/04 cells were untreated or treated with 10 ng/ml TGF-β2 for 48 h. (a) The FEZF1-AS1 level in SRA01/04 cells was detected by qRT-PCR. (b) The viability of human lens epithelial cells (HLECs, SRA01/04 cells) was detected by CCK-8 assay. (c) SRA01/04 cells were stained with EdU (red) and DAPI (blue). Scale bar = 50 μm. (c) The bar chart shows the average ratio of EdU-stained cells to DAPI-stained cells. CDK2, CDK4, CDK6, and cyclin D1 protein levels were detected by western blot (e) and band density analysis (f). (g) Untreated SRA01/04 cells or SRA01/04 cells treated with TGF-β2 for 0 h, 24 h, 36 h, or 48 h were subjected to a wound healing assay to detect migration. Scale bar = 50 μm. (h) The average relative cell migration ratio compared to 0 h was calculated. n = 5 in each group. , vs blank control group.

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